Cytokinins and pH on the in vitro culture of strawberry shoots
DOI:
https://doi.org/10.29312/remexca.v17i3.4322Keywords:
Fragaria x ananassa, benzylaminopurine, shoot proliferation, thidiazuronAbstract
Conventional strawberry propagation is performed using runners, which may limit the number and health of plants. The present work assessed the effects of different culture media variants on shoot proliferation in vitro, shoot size and root formation. Seventy-seven variants of culture media were prepared with inorganic salts of the Murashige and Skoog (MS) formulation, different concentrations of two cytokinins, benzylaminopurine, BAP (0, 0.5 and 1 mg L-1) and thidiazuron TDZ (0, 0.5 and 1 mg L-1) and different pH levels (5, 5.5 and 5.8). Data was taken on the number of shoots, the number of leaves and root formation. The data were subjected to analysis of variance and Tukey’s test (0.05). The highest number of shoots (11), with up to 57 leaves, was obtained in the culture medium with 0.5 mg L-1 TDZ, 0 mg L-1 BAP and a pH of 5.5. The lowest numbers of shoots (2.3), with 18 leaves, were obtained in CM without TDZ, 0.5 mg L-1 BAP and a pH of 5.5. In environments with only BAP, the new shoots were taller and formed adventitious roots. TDZ inhibited root formation. It is concluded that the presence of TDZ in CMs stimulated more axillary buds to sprout, while BAP promoted the development of axillary buds but with a lesser effect than that of TDZ.
Downloads
References
Adak, N.; Kaynak, L.; Pekmezci, M. and Gubbuk, H. 2009. Effect of thidiazuron and indole-3-acetic acid on in vitro shoot proliferation of strawberry (Fragaria × ananassa Duch.). Acta Horticulturae. 829:323-328. https://doi.org/10.17660/ActaHortic.2009.829.46. DOI: https://doi.org/10.17660/ActaHortic.2009.829.46
Al-Madhagi, A. 2023. In vitro propagation of strawberry (Fragaria × ananassa Duch.) using cytokinins. Bioagro. 37(1):123-134.
Benavides-Mendoza, A.; González-Moscoso, M.; Ojeda-Barrios, D. L. y Fuentes-Lara, L. O. 2021. Bioestimulación y toxicidad: Nanotecnología para la promoción y protección del crecimiento de las plantas. Capítulo 14. 245-268 pp. https://onlinelibrary.wiley.com/doi/abs/10.1002/9781119745884.ch14.
Boxus, P. 1983. Propagation of strawberry plants by in vitro culture. Acta Horticulturae. 131:35-44.
Cappelletti, R.; Sabbadini, S. and Mezzetti, B. 2016. The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars. Scientia Horticulturae. 207(1):117-124. https://doi.org/10.1016/j.scienta.2016.05.016. DOI: https://doi.org/10.1016/j.scienta.2016.05.016
Capocasa, F.; Balducci, F. and Marcellini, M. 2019. Comparación del comportamiento en vivero, el rendimiento de las plantas en campo y la calidad del fruto de plantas madre de fresa propagadas in vitro e in vivo. Plant Cell Tiss Organ Cult. 136:65-74. https://doi.org/10.1007/s11240-018-1492-8. DOI: https://doi.org/10.1007/s11240-018-1492-8
Cuazitl-Flores, M. F. y Núñez-Palenius, H. G. 2017. Establecimiento y micropropagación de fresa (Fragaria × ananassa Duch.) en un sistema de inmersión temporal (SIT). Revista Mexicana de Ciencias Agrícolas. 8(4):799-812.
Enríquez-Valle, J. R. 2008. La propagación y crecimiento de agaves. Primera Edición. Fundación produce Oaxaca e Instituto Tecnológico del Valle de Oaxaca. 48 p.
FAO. 2023. Guía práctica para la aplicación de las normas para bancos de germoplasma de recursos fitogenéticos para la alimentación y la agricultura: conservación mediante cultivo in vitro. Comisión de Recursos Genéticos para la Alimentación y la Agricultura. Roma. 120 p. https://doi.org/10.4060/cc0025es. DOI: https://doi.org/10.4060/cc0025es
Gonzales-Arteaga, J. J.; Rodríguez-Layza, J. Romero-Rivas, L. C.; Párraga-Quintanilla, A. and Olivera-Soto, J. A. 2023. Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties. Agroindustrial Science. 13(2):93-102. http://doi.org/10.17268/agroind.sci.2023.02.05. DOI: https://doi.org/10.17268/agroind.sci.2023.02.05
Guevara-Matus, K.; Loría-Quirós, C. L. y Granados-Montero, M. 2023. Efecto de la vernalización sobre la producción de estolones de fresa. Revista Mexicana de Ciencias Agrícolas. 14(1):129-137. DOI: https://doi.org/10.29312/remexca.v14i1.3187
Kirschbaum, D. S. 2021. Características botánicas, fisiología y tipos de variedades de fresas. Cultivo, poscosecha, procesado y comercio de berries. Horticultura y Poscosecha. 103-116 pp. ISBN: 978-84-16909-47-6.
Miguel-Luna, M. E.; Enríquez-Valle, J. R.; Velasco-Velasco, V. A.; Villegas-Aparicio, Y.; Carrillo-Rodríguez, J. C. y Rodríguez-Ortiz, G. 2013. Composición del medio de cultivo y la incubación para enraizar brotes de agave. Revista Mexicana de Ciencias Agrícolas. 6(14):1151-1159. DOI: https://doi.org/10.29312/remexca.v0i6.1279
Miguel-Luna, M. E.; Enríquez-Valle, J. R.; Santos-Romero, R. B. y Rodríguez-Ortiz, G. 2022. La morfogénesis en la propagación asexual, con énfasis en cultivo de tejidos vegetales. Revista Mexicana de Agroecosistemas. 9(1):137-147.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiología Plantarum. 15(3):473-479. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Neri, J.; Meléndez-Mori, R.; Tejada-Alvarado, K.; Vilca-Valqui, M.; Huamán-Huamán, J.; Oliva, C. y Goñas, J. 2022. An optimized protocol for micropropagation and acclimatization of strawberry (Fragaria × ananassa Duch.) Variety ‘Aroma’. Agronomy. 12(4):968. https://doi.org/10.3390/agronomy12040968. DOI: https://doi.org/10.3390/agronomy12040968
Pasternak, T. P. and Steinmacher, D. 2024. Plant growth regulation in cell and tissue culture in vitro. Plants. 13(2):327-357. https://doi.org/10.3390/plants13020327. DOI: https://doi.org/10.3390/plants13020327
Pérez-Molphe, B. E. 2007. Cultivo de tejidos vegetales. Universidad Autónoma de Aguascalientes. 21-63 pp. ISBN 968-625-962-7.
Pillco-Tancara, H. C. y Quezada-Portugal, J. Á. N. 2017. Efecto del tidiazurón y ácido indolbutírico en la propagación in vitro de dos variedades de frutilla (Oso Grande y Sweet Charlie) a partir de secciones foliares. Journal of the Selva Andina Research Society. 8(1):53-64. http://www.scielo.org.bo/pdf/jsars/v8n1/v8n1-a05.pdf. DOI: https://doi.org/10.36610/j.jsars.2017.080100069
Romero-Rivas, L. C.; Gonzales-Arteaga, J. J.; Rodríguez-Layza, J.; Párraga-Quintanilla, A. y Olivera-Soto, J. A. 2023. Multiplicación y enraizamiento in vitro de Fragaria x ananassa Duch. Var. ‘Aromas’. Revista de Investigaciones Altoandinas-Journal of High Andean Research. 25(4):205-212.https://doi.org/10.18271/ria.2023.563. DOI: https://doi.org/10.18271/ria.2023.563
Ruíz-Anchondo, T.; Martínez, J. A.; Carrillo-Castillo, T.; Parra-Quezada, R. Á.; Ojeda Barrios, D. L. y Hernández-Rodríguez, A. 2018. Establecimiento in vitro de dos cultivares liberados de frutillas: fresa y frambuesa. Revista Mexicana de Ciencias Agrícolas. 9(4):799-812. DOI: https://doi.org/10.29312/remexca.v9i4.1397
SAS Institute Inc. 2022. SAS® 9.4 software [Computer software]. Cary, NC: SAS Institute Inc.
Venegas Tarancón, R.; Rodríguez, J.; Rojas, J. y Díaz, C. 2024. Uso de fitohormonas sintéticas y extractos vegetales en el cultivo in vitro de fresa (Fragaria × ananassa). Acta Agronómica. 73(2):143-152. https://doi.org/10.15446/acagro.v73n2.110143.
Wang, F.; Li, Y.; Pang, Y.; Hu, J.; Kang, X. and Qian, C. 2025. Thidiazuron enhances strawberry shoot multiplication by regulating hormone signal transduction pathways. International Journal of Molecular Sciences. 26(9):4060. https://doi.org/10.3390/ijms26094060. DOI: https://doi.org/10.3390/ijms26094060
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Revista Mexicana de Ciencias Agrícolas
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The authors who publish in Revista Mexicana de Ciencias Agrícolas accept the following conditions:
In accordance with copyright laws, Revista Mexicana de Ciencias Agrícolas recognizes and respects the authors’ moral right and ownership of property rights which will be transferred to the journal for dissemination in open access. Invariably, all the authors have to sign a letter of transfer of property rights and of originality of the article to Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) [National Institute of Forestry, Agricultural and Livestock Research]. The author(s) must pay a fee for the reception of articles before proceeding to editorial review.
All the texts published by Revista Mexicana de Ciencias Agrícolas —with no exception— are distributed under a Creative Commons License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned.
The author(s) can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Revista Mexicana de Ciencias Agrícolas (for example include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Revista Mexicana de Ciencias Agrícolas.
For all the above, the authors shall send the Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a PDF file to: revista_atm@yahoo.com.mx; cienciasagricola@inifap.gob.mx; remexca2017@gmail.
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 International license.
