Fertigation and Azospirillum in the nutrition of Agave angustifolia vitroplants in a nursery
DOI:
https://doi.org/10.29312/remexca.v17i3.4325Keywords:
nutrient analysis, nutrient content, nutrient solution, plant growth-promoting bacteria, plant micropropagationAbstract
Humanity seeks to increase the productivity and quality of crops that provide basic or economic benefits. The work aimed to evaluate the development of Agave angustifolia vitroplants fertigated with different nutrient aliquots and inoculated with Azospirillum brasilense from 2023 to 2024. In an experiment with a 3 × 2 × 3 factorial experimental design, the factors were: 1) initial plant size (small, medium, and large); 2) inoculation with the bacterium A. brasilense and without inoculation; and 3) concentrations of the Steiner nutrient solution (SN) (0, 75 and 100%). After 20 months, morphological characteristics and the amount of nutrients in the leaves were evaluated. Plants with a larger initial size maintained differences in growth compared to medium and small plants. The plants fertigated with 100% SN had a height of 51 cm (18.6%), a rosette diameter of 85.8 cm (25.8%) and a stem diameter of 9.6 cm (65.5%). Their largest leaf was 66 cm (13.7%), and their leaves contained 6 987 ppm N (9.6%), 1 775 ppm P (11%), and 3 093 ppm K (-25.8%) compared to plants irrigated only with water. The plants inoculated with the bacterium had more leaves, a larger rosette diameter, greater leaf length, greater root volume and greater stem and root dry weight than non-inoculated plants. The micropropagated A. angustifolia plants that were fertigated in a nursery and in parallel, inoculated with A. brasilense reached adequate sizes for transplantation in the field.
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Alcántar, G. G. y Sandoval, V. M. 1999. Manual de análisis químico de tejido vegetal. Sociedad Mexicana de la Ciencia del Suelo, AC. Chapingo, México. Publicación especial 10. 156 p.
Bashan, Y. and Holguin, G. 1998. A proposal for the division of “plant growth-promoting rhizobacteria” into two classifications: biocontrol-plant growth-promoting bacteria and plant growth-promoting bacteria. Soil Biology and Biochemistry. 30(9):1225-1228. DOI: https://doi.org/10.1016/S0038-0717(97)00187-9
Cruz-García, H.; Campos-Ángeles, G. V.; Enríquez-Valle, J. R.; Rodríguez-Ortiz, G. y Velasco-Velasco, V. A. 2019. Desarrollo de plantas micropropagadas de Agave americana var. Oaxacensis durante su aclimatización en invernadero. Revista Mexicana de Ciencias Agrícolas. 10(7):1491-1503. https://doi.org/10.29312/remexca.v10i7.1625. DOI: https://doi.org/10.29312/remexca.v10i7.1625
Domínguez-Duarte, C. F.; Cecato, U.; Trento-Biserra, T.; Mamédio D. y Galbeiro, S. 2019. Azospirillum spp. en gramíneas y forrajeras. Revista Mexicana de Ciencias Pecuarias. 11(1):223-240. https://doi.org/10.22319/rmcp.v11i1.4951. DOI: https://doi.org/10.22319/rmcp.v11i1.4951
Enríquez-Valle, J. R.; Rodríguez-Ortiz, G.; Ruiz-Luna, J.; Pacheco-Ramírez. A. J. y Vázquez-Vázquez L. 2018. Crecimiento y condición nutrimental en plantas micropropagadas de A. angustifolia abonadas y fertirrigadas en vivero. Revista Mexicana de Agroecosistemas. 5(2):106-115.
Enríquez-Valle, J. R.; Chávez-Cruz, I. L.; Rodríguez-Ortiz, G. y Campos-Ángeles, G. V. 2023. Vitroplantas de Agave angustifolia Haw. obtenidas en ambientes de incubación contrastantes, aclimatadas en diferentes sustratos. Revista Fitotecnia Mexicana. 46(3):291-298. Doi: https://doi.org/10.35196/rfm.2023.3.291. DOI: https://doi.org/10.35196/rfm.2023.3.298
García, M. A. y Oberschelp, G. P. J. 2015. Fertilización de Eucalyptus grandis en vivero: calidad de las plantas y comportamiento a campo. XXIX Jornadas forestales de entre ríos. 5 p. http://hdl.handle.net/20.500.12123/17698.
Glick, B. R. 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiology Research. 169:30-39. https://doi.org/10.1016/j.micres.2013.09.009. DOI: https://doi.org/10.1016/j.micres.2013.09.009
Jarquín-Rosales, D.; Enríquez-Valle, J. R.; Alpuche-Osorno, J. J.; Rodríguez-Ortiz, G.; Martin, M. P. and Campos-Angeles, G. V. 2022. The effects of fertirrigation and Azospirillum brasilence inoculation on photosynthetic compounds of Agave angustifolia. Australian Journal of Crop Science. 16(01):162-168. Doi: 10.21475/ajcs.22.16.01.p3280. DOI: https://doi.org/10.21475/ajcs.22.16.01.p3280
Jarquín-Rosales, D.; Enríquez-Valle, J. R.; Alpuche-Osorno, J. J.; Rodríguez-Ortiz, G.; Campos-Ángeles, G. V and Morales, I. 2023. Agave angustifolia bulbil growth in differents substrates, with doses of fertirrigation and inoculation with Azospirillum brasilense. Ciencia Rural. 53(3):e20210863. http://doi.org/10.1590/0103-8478cr20210863. DOI: https://doi.org/10.1590/0103-8478cr20210863
Lavenus, J.; Goh, T.; Roberts, I.; Guyomarc’h, S.; Lucas, M.; Smet, I. and Fukaki, H. 2013. Lateral root development in arabidopsis: fifty shades of auxin. Trends Plant Science. 18(8):450-458. https://doi.org/10.1016/j.tplants.2013.04.006. DOI: https://doi.org/10.1016/j.tplants.2013.04.006
Luna-Luna, J. S.; Enríquez-Valle, J. R.; Rodríguez-Ortiz, G.; Carrillo-Rodríguez, J. C. y Velasco-Velasco, V. A. 2017. Anatomía y morfología de plantas micropropagadas-aclimatadas de Agave potatorum zucc. fertirrigadas en vivero. Revista Fitotecnia Mexicana. 40(4):491-494. https://doi.org/10.35196/rfm.2017.4.491-494. DOI: https://doi.org/10.35196/rfm.2017.4.491-494
Muñoz-Flores, H. J.; Sáenz-Reyes, J. T.; Coria-Avalos, V. M.; García-Magaña, J. J.; Hernández-Ramos, J. y Manzanilla-Quijada, G. E. 2015. Calidad de planta en el vivero forestal La Dieta, Municipio Zitácuaro, Michoacán. Revista Mexicana de Ciencias Forestales. 6(27):72-89. DOI: https://doi.org/10.29298/rmcf.v6i27.282
Okon, Y. and Vanderleyden, J. 1997. Root-associated Azospirillum species can stimulate plants. Applied Environment Microbiology. 63(7):366-370.
Olvera-Vargas, L. A. J.; Pardo-Núñez, N.; Aguilar-Rivera, D. y Contreras-Medina. I. 2022. Detección de Agave angustifolia y Agave cupreata con técnicas geomáticas en Guerrero, México. Ciencia y Tecnología Agropecuaria. 23(2):e2241. https://doi.org/10.21930/rcta.vol23-num2-art:2241. DOI: https://doi.org/10.21930/rcta.vol23_num2_art:2241
Pérez-Santiago, R.; Enríquez-Valle, J. R.; Castañeda-Hidalgo, E.; Velasco-Velasco, A. V.; Rodríguez-Ortiz, G. y Campos-Ángeles, G. V. 2014. Dosis de fertirriego durante la aclimatación de plantas de Agave americana Micropropagadas. Revista Mexicana de Agroecosistemas. 1(1):20-27.
Ríos-Ramírez, S. C.; Enríquez-Valle, J. R.; Rodríguez-Ortiz, G.; Ruíz-Luna, J. y Velasco-Velasco, V. A. 2021. El crecimiento de Agave angustifolia Haw. con relación a la condición nutrimental. Revista Mexicana de Ciencias Agrícolas. 12(5):865-873. https://doi.org/10.29312/remexca.v12i5.2638. DOI: https://doi.org/10.29312/remexca.v12i5.2638
Sánchez-Mendoza, S.; Bautista-Aparicio, G. and Bautista-Cruz, A. 2022. Inorganic fertilization improves Agave potatorum Zucc growth and nutrition. International Journal of Agriculture and Natural Resources. 49(3):147-156. Doi: 10.7764/ijanr.v 49i3.2338. DOI: https://doi.org/10.7764/ijanr.v49i3.2338
SAS. 2011. Institute. SAS/STAT User’s Guide. Ver. 9.3. SAS Institute, Inc. Cary, NC. 8621. http://support.sas.com/documentation/cdl/en/statug/63962/PDF/default/statug.pdf.
Shu, K.; Liu, X. D.; Xie, Q. and He, Z. H. 2016. Two faces of one seed: hormonal regulation of dormancy and germination. Molecular Plant. 9:34-45. https://doi.org/10.1016/j.molp.2015.08.010. DOI: https://doi.org/10.1016/j.molp.2015.08.010
SIAP 2019. Servicio de Información Agroalimentaria y Pesquera. Secretaría de Agricultura y Desarrollo Rural. Ciudad de México. https://nube.siap.gob.mx/cierreagricola/.
Steiner, A. A. 1984. The universal nutrient solution. In: proceedings of IWOSC 1984 6 the International Congress on Soilless Culture. Wageningen, the netherlands. 633-650 pp.
Zúñiga-Estrada, L.; Rosales-Robles, E.; Yáñez-Morales, M. J. y Jacques-Hernández, C. 2018. Características y productividad de una planta MAC, Agave tequilana desarrollada con fertigación en Tamaulipas, México. Revista Mexicana de Ciencias Agrícolas. 9(3):553-564. https://doi.org/10.29312/remexca.v9i3.1214. DOI: https://doi.org/10.29312/remexca.v9i3.1214
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