Culture media for seed germination and seedling growth of Laelia autumnalis

Authors

  • Marcela Cabañas-Rodríguez Facultad de Ciencias Agropecuarias. Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México. CP. 62209 https://orcid.org/0009-0004-8405-9233
  • María Andrade-Rodríguez Facultad de Ciencias Agropecuarias. Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México. CP. 62209 https://orcid.org/0000-0003-0757-742X
  • Oscar Gabriel Villegas-Torres Facultad de Ciencias Agropecuarias. Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México. CP. 62209 https://orcid.org/0000-0001-9885-3906
  • José Antonio Chávez-García Facultad de Ciencias Agropecuarias. Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México. CP. 62209 https://orcid.org/0000-0002-1480-8401
  • Iran Alia-Tejacal Facultad de Ciencias Agropecuarias. Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México. CP. 62209 https://orcid.org/0000-0002-2242-2293
  • Porfirio Juárez-López Facultad de Ciencias Agropecuarias. Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México. CP. 62209 https://orcid.org/0000-0002-4241-1110

DOI:

https://doi.org/10.29312/remexca.v16i6.3811

Keywords:

day of the dead orchid, MS medium, orchid seedlings

Abstract

The orchid Laelia autumnalis is endemic to Mexico and is under special protection. In vitro culture has contributed to improving the propagation of orchids from seeds. In order to micropropagate this species, an experiment was established in 2023 to evaluate eight culture media: Murashige and Skoog (MS at 100, 75, 50 and 25%), Knudson C, Dalla Rosa and Laneri, Vacint and Went, and Lindemann; all media were supplemented with 0.5 mg L-1 thiamine HCl, 100 mg L-1 myo-inositol and 3% sucrose to determine the best medium for germination of this species. Seeds were disinfected with 0.3% sodium hypochlorite (NaClO) and sown in the media in a completely randomized design with 10 replications per treatment. Germination start and percentage were recorded; seedling height, leaf length and number, root number and length, and pseudobulb diameter were evaluated at 90 days. The 25 and 50% MS media generated a higher percentage of germination (84.57 and 72.54%, respectively), which began 29 days after seeding. Growth in height (5.6 mm), leaf length (4.54 mm) and root length (5.2 mm) were higher in the MS medium at 50%, where seedlings were green. It was concluded that the best culture medium for germination and growth of L. autumnalis seedlings was the MS at 50% concentration.

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Published

2025-10-01

How to Cite

Cabañas-Rodríguez, Marcela, María Andrade-Rodríguez, Oscar Gabriel Villegas-Torres, José Antonio Chávez García, Iran Alia-Tejacal, and Porfirio Juárez-López. 2025. “Culture Media for Seed Germination and Seedling Growth of Laelia Autumnalis”. Revista Mexicana De Ciencias Agrícolas 16 (6). México, ME:e3811. https://doi.org/10.29312/remexca.v16i6.3811.

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Vol. 16 No. 6 (2025)

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