Evaluation of bioreactors with and without air injection for the micropropagation of Vanilla planifolia G. Jackson

Authors

  • Clara Anabel Arredondo-Ramírez Postgrado de Recursos Genéticos y Productividad-Fruticultura-Campus Montecillo-Colegio de Postgraduados. Carretera Mexico-Texcoco km 36.5, Montecillo, Texcoco, Estado de México México. CP. 56230. Tel. 55 58045900
  • Gregorio Arellano-Ostoa Postgrado de Recursos Genéticos y Productividad-Fruticultura-Campus Montecillo-Colegio de Postgraduados. Carretera Mexico-Texcoco km 36.5, Montecillo, Texcoco, Estado de México México. CP. 56230. Tel. 55 58045900
  • Sergio Humberto Chávez-Franco Postgrado de Recursos Genéticos y Productividad-Fruticultura-Campus Montecillo-Colegio de Postgraduados. Carretera Mexico-Texcoco km 36.5, Montecillo, Texcoco, Estado de México México. CP. 56230. Tel. 55 58045900
  • Eduardo García-Villanueva Postgrado de Recursos Genéticos y Productividad-Fruticultura-Campus Montecillo-Colegio de Postgraduados. Carretera Mexico-Texcoco km 36.5, Montecillo, Texcoco, Estado de México México. CP. 56230. Tel. 55 58045900
  • María del Carmen López-Reyna Postgrado en Socioeconomía, Estadística e Informática-Economía. Campus Montecillo-Colegio de Postgraduados. Carretera Mexico-Texcoco km 36.5, Montecillo, Texcoco, Estado de México, México. Tel. 55 58045900
  • José Humberto Caamal-Velázquez Bioprospección y Sustentabilidad Agrícola en Trópico-Campus Campeche-Colegio de Postgraduados. Carretera Haltunchén-Edzná, Champotón, Campeche. México. CP. 24450

DOI:

https://doi.org/10.29312/remexca.v16i6.3804

Keywords:

liquid media, mechanical, micropropagation, Rita®, vanilla

Abstract

The species Vanilla planifolia G. Jackson has a high commercial value in the food, pharmaceutical and cosmetic industries. Temporary immersion systems or bioreactors allow for faster and more controlled in vitro propagation under laboratory conditions. Nonetheless, due to the high costs of commercial bioreactors, such as the Rita® model, one of the most widely used for the micropropagation of several plant species, cheaper alternatives are sought. In vitro multiplication of vanilla was carried out in two types of temporary immersion bioreactors in order to evaluate the efficiency of a mechanical bioreactor without air injection compared to a Rita® bioreactor that uses air injection; the research was conducted between 2023 and 2024. The semi-solid culture system was used as a control. After 30 days of culture, the following physiological variables were analyzed: number of shoots, number of leaves, shoot length, and growth index; likewise, biochemical variables, such as total contents of chlorophylls α and β and phenol and carbohydrate contents, were quantified. The results obtained indicated that the BWA bioreactor was statistically equal (p≤ 0.05) to the Rita® bioreactor in the variables of number and length of shoots. Both were statistically different (p≤ 0.05) from the semi-solid system in most of the variables assessed. This suggests that the use of a mechanical bioreactor without air injection can be used as an alternative for the micropropagation of various species due to its low cost.

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References

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Published

2025-10-07

How to Cite

Arredondo-Ramírez, Clara Anabel, Gregorio Arellano-Ostoa, Sergio Humberto Chávez-Franco, Eduardo García-Villanueva, María del Carmen López-Reyna, and José Humberto Caamal-Velázquez. 2025. “Evaluation of Bioreactors With and Without Air Injection for the Micropropagation of Vanilla Planifolia G. Jackson”. Revista Mexicana De Ciencias Agrícolas 16 (6). México, ME:e3804. https://doi.org/10.29312/remexca.v16i6.3804.

Issue

Vol. 16 No. 6 (2025)

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Articles

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