Seed disinfection and culture media in the in vitro germination and growth of seedlings of Eysenhardtia polystachya (Ortega)

Authors

  • Norma Angélica Lorenzo-Barrera Posgrado en Ciencias Agropecuarias y Desarrollo Rural-Facultad de Ciencias Agropecuarias-Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México. CP. 62209. Tel. 777 3297900 https://orcid.org/0000-0003-4742-6191
  • María Andrade-Rodríguez Posgrado en Ciencias Agropecuarias y Desarrollo Rural-Facultad de Ciencias Agropecuarias-Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México. CP. 62209. Tel. 777 3297900 https://orcid.org/0000-0003-0757-742X
  • Oscar Gabriel Villegas-Torres Posgrado en Ciencias Agropecuarias y Desarrollo Rural-Facultad de Ciencias Agropecuarias-Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México. CP. 62209. Tel. 777 3297900 https://orcid.org/0000-0001-9885-3906
  • Teresa de Jesús Rodríguez Rojas Universidad Autónoma del Estado de Morelos-Escuela de Estudios Superiores de Xalostoc. Nicolas Bravo s/n, Jaloxtoc, Morelos, México. Tel. 735 2802240 https://orcid.org/0000-0003-3262-9172
  • Erika Román Montes de Oca Universidad Autónoma del Estado de Morelos-Escuela de Estudios Superiores de Xalostoc. Nicolas Bravo s/n, Jaloxtoc, Morelos, México. Tel. 735 2802240 https://orcid.org/0000-0002-9490-6422

DOI:

https://doi.org/10.29312/remexca.v16i5.3744

Keywords:

disinfection method, kidneywood, MS medium, WPM medium

Abstract

Eysenhardtia polystachya is a plant prized for the strength of its wood and medicinal use. It is propagated by seed; nevertheless, low germination and attack by insects and fungi in natural conditions limit its availability, which makes it highly vulnerable. In vitro culture allows the number of individuals to increase rapidly. The objective was to evaluate the effect of mineral salts of Murashige and Skoog and Woody Plant Medium culture media, both in concentration of 50, 75 and 100% (macro and micronutrients), in combination with two methods of seed disinfection. E. polystachya seeds collected in November 2021 in Tlayacapan Morelos were used; these were disinfected with silver nanoparticles, applied once or twice, and then seeded in vitro. The experimental design was completely randomized with factorial arrangement of treatments, with 10 replications of five seeds. Double application of silver nanoparticles generated 98.3% of aseptic seeds, compared to applying them once (86.7%). Plant height was also 34.5% higher. The MS medium generated better germination in the three concentrations (90 to 99%). Root length and dry matter were more abundant in the MS medium at 50%. It was concluded that it is convenient to use silver nanoparticles twice to disinfect E. polystachya seeds and to use the 50% MS culture medium for germination and seedling growth.

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References

Andrade-Rodríguez, M.; Vargas-Araujo, J.; Villegas-Torres, O. G.; López-Martínez, V.; Guillen-Sánchez, D. y Alia-Tejacal, I. 2015. Germinación de semillas y crecimiento de plántulas de Cattleya (Brassolaeliocattleya) in vitro. Interciencia. 40(8):549-553. https://www.interciencia.net/wp-content/uploads/2017/10/549-c-andrades5.pdf.

Bello-Bello, J. y Spinoso-Castillo, J. 2022. Utilización de nanopartículas de plata en la micropropagación de plantas. Revista Interdisciplinaria en nanociencias y nanotecnología. 16(30):1-14. https://doi.org/10.22201/ceiich.24485691e.2023.30.69692.

Beltrán-Rodríguez, L.; Manzo-Ramos, F.; Maldonado-Almanza, B.; Martínez-Ballesté, A. and Blancas, J. 2017. Wild medicinal species traded in the Balsas Basin, Mexico: risk analysis and recommendations for their conservation. Journal of Ethnobiology. 37(4):743-764. https://doi.org/10.2993/0278-0771-37.4.743.

Beltrán-Rodríguez, L.; Maldonado-Almanza, B.; Cristians, S.; Blancas, J.; Sierra-Huelz, A. y Bye, R. 2020. Las cortezas como productos forestales no maderables en México: Análisis nacional y recomendaciones para su aprovechamiento sostenible. Instituto de Biología. Universidad Nacional Autónoma de México (UNAM). Consejo Nacional de Ciencia y Tecnología. 47 p.

Bernabé-Antonio, A.; Maldonado-Magaña, A.; Ramírez-López, C. B.; Salcedo-Pérez, E.; Meza-Contreras, J. C.; González-García, Y.; López-Dellamary, T. and Cruz-Sosa, F. 2017. Establishment of callus and cell suspension cultures of Eysenhardtia polystachya (Ortega) and fungistatic activity of their extracts. South African Journal of Botany. 112:40-47. https://doi.org/10.1016/j.sajb.2017.05.023.

Bernabé-Antonio, A.; Sánchez-Sánchez, A.; Romero-Estrada, A.; Meza-Contreras, J. C.; Silva-Guzmán, J. A.; Fuentes-Talavera, F. J.; Hurtado-Díaz, I. and Cruz-Sosa, F. 2021. Establishment of a cell suspension culture of Eysenhardtia platycarpa: Phytochemical screening of extracts and evaluation of antifungal activity. Plants. 10(2):1-21. https://doi.org/10.3390/plants10020414.

Campos, R. J.; Arteaga, M. C.; Campos, S. R.; Chico, J. R. y Cerna, R. L. 2020. Establecimiento de un protocolo de desinfección y micropropagación in vitro de “caoba” Swietenia macrophylla King (Meliaceae). Arnaldoa. 27(1):141-156. http://doi.org/10.22497/arnaldoa.271.27107.

Chávez-García, J. A.; Andrade-Rodríguez, M.; Bello-Bello, J. J.; Rueda-Barrientos, M. C.; Guillén-Sánchez, D. y Sainz-Aispuro, M. J. 2020. Nanopartículas de plata en el establecimiento in vitro de ápices de gladiolo. Revista Fitotecnia Mexicana. 43(4-A):557-564. https://doi.org/10.35196/rfm.2020.4-A.557.

Gelviz-Gelvez, S. M.; Pavón, N. P.; Flores, J.; Barragán, F. y Paz, H. 2020. Germinación de siete especies de arbustos en el centro semiárido de México: efecto de la sequía y el tamaño de la semilla. Botanical Sciences. 98(3):464-472. http://doi.org/10.17129/botsci.2537.

Haida, Z.; Nakasha, J. J. and Hakiman, M. 2020. In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah Snake Grass). Plants. 9(8):1-17. https://doi.org/10.3390/plants9081030.

Lorenzo-Barrera, N. A.; Andrade-Rodríguez, M.; Villegas-Torres, O. G.; Román-Montes, E.; Sotelo-Nava, H.; Rodríguez-Rojas, T. J. y Suárez-Rodríguez, R. 2023. Usos del palo dulce Eysenhardtia polystachya (Ort.) Sarg., en cuatro municipios del estado de Morelos, México. Polibotánica. 55(28):161-177. http://doi.org/10.18387/polibotanica.55.11.

Lorenzo-Barrera, N. A.; Andrade-Rodríguez, M.; Villegas-Torres, O. G. and Sotelo-Nava, H. 2024. Pre-germination treatments on kidneywood (Eysenhardtia polystachya) seeds. Revista Ciência Agronômica. 55:e20238765. https://doi.org/10.5935/1806-6690.20230067.

McCown, B. H. and Lloyd, G. 1981. Woody Plant Medium (WPM)-a mineral nutrient formulation for microculture of Woody Plant Species. HortScience. 16(4):453-453.

Martín, R. M. H.; Ibarra, F. A.; Moreno, M. S.; Hernández, J. E. y Retes, R. 2021. Costo beneficio asociado con la cosecha de semilla de palo dulce y sitiporo en la región central de Sonora, México. Revista Mexicana de Agronegocios. 48(1):754-764. https://www.redalyc.org/articulo.oa?id=14167610015.

Martínez-Villegas, Y. M.; Andrade-Rodríguez, M.; Colinas-León, T.; Villegas-Torres, O. G.; Castillo-Gutiérrez, A. y Alia-Tejacal, I. 2015. Efecto de las sales inorgánicas del medio de cultivo en el crecimiento de pascuita (Euphorbia leucocephala Lotsy). Revista Fitotecnia Mexicana. 38(4):369-374. https://www.scielo.org.mx/scielo.php?pid=S0187-73802015000400004&script=sci-abstract&tlng=pt.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiology Plantarum. 15(3):473-497.

Núñez-Cruz, A.; Meave, J. A. and Bonfil, C. 2018. Reproductive phenology and seed germination in eight tree species from a seasonally dry tropical forest of Morelos, Mexico: Implications for community-oriented restoration and conservation. Tropical Conservation Science. 11(1):1-14. https://doi.org/10.1177/1940082917749946.

Pinedo-Panduro, M.; Alves-Chagas, E.; Freitas-Luz, F.; Cardoso-Chagas, P.; Panduro-Tenazoa, N. M.; Bardales-Lozano, R.; Abanto-Rodríguez, C.; Paredes-Dávila, E. y Collazos-Saldaña, H. 2022. Efecto de las sales de los medios de cultivo Murashige & Skoog y Knudson sobre el establecimiento in vitro de Epidendrum schomburgkii Lindl. Ciencia y Tecnología Agropecuaria. 23(3):2526, 1-14. https://doi.org/10.21930/rcta.vol23-num3-art:2526.

Ramírez-Mosqueda, M. A.; Sánchez‑Segura, L. and Hernández‑Valladolid, S. L. 2020. The influence of silver nanoparticles on a common contaminant isolated during the establishment of Stevia rebaudiana Bertoni culture. Plant Cell Tissue and Organ Culture. 143(3):609‑618. https://doi.org/10.1007/s11240‑020‑01945‑9.

Raya-Pérez, J. C.; Aguirre-Mancilla, C. L.; Covarrubias-Prieto, J.; Ramírez-Pimentel, G. y Iturriaga, G. 2020. El osmoacondicionamiento de las semillas agrícolas. Ciencia y Tecnología Agropecuaria. 8(1):1-8. https://www.somecta.org.mx/rev-ciencia-y-tecnol-agrop-mexico/revistas/2020-1/2020-1-1/.

SAS Institute Inc. 1995. SAS/STAT User’s guide, Version 9,2. SAS Institute. Cary, NC.

Spinoso-Castillo, J. L.; Chávez-Santoscoy, R. A.; Bogdanchikova, N.; Pérez-Sato, J. A.; Morales-Ramos, V. and Bello-Bello, J. J. 2017. Antimicrobial and hormetic effects of silver nanoparticles on in vitro regeneration of vanilla (Vanilla planifolia Jacks. ex Andrews) using a temporary immersion system. Plant Cell Tissue and Organ Culture. 129(2):195-207. https://doi.org/10.1007/s1124001711698.

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Published

2025-08-05

How to Cite

Lorenzo-Barrera, Norma Angélica, María Andrade-Rodríguez, Oscar Gabriel Villegas-Torres, Teresa de Jesús Rodríguez Rojas, and Erika Román Montes de Oca. 2025. “Seed Disinfection and Culture Media in the in Vitro Germination and Growth of Seedlings of Eysenhardtia Polystachya (Ortega)”. Revista Mexicana De Ciencias Agrícolas 16 (5). México, ME:e3744. https://doi.org/10.29312/remexca.v16i5.3744.

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Vol. 16 No. 5 (2025)

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