Genetic diversity of Capsicum pubescens by functional genomic markers of CYP450

Authors

  • Ana Karen Escalera-Ordaz Facultad de Agrobiología ‘Presidente Juárez’-Unidad de Investigaciones Avanzadas en Agrobiotecnología-Universidad Michoacana de San Nicolás de Hidalgo. Paseo Lázaro Cárdenas esquina con Berlín S/N, Emiliano Zapata, Uruapan, Michoacán, México. CP. 60170 Tel. 452 5236474
  • Héctor Guillén-Andrade Facultad de Agrobiología ‘Presidente Juárez’-Unidad de Investigaciones Avanzadas en Agrobiotecnología-Universidad Michoacana de San Nicolás de Hidalgo. Paseo Lázaro Cárdenas esquina con Berlín S/N, Emiliano Zapata, Uruapan, Michoacán, México. CP. 60170 Tel. 452 5236474
  • Pedro Antonio López Colegio de Postgraduados-Campus Puebla. Carretera federal México-Puebla km 125.5, Santiago Momoxpan, San Pedro Cholula, Puebla, México. CP. 72760. Tel. 222 2851442
  • Luis Mario Tapia Vargas Campo Experimental Uruapan-INIFAP. Avenida Latinoamericana 1101. Colonia Revolución, Uruapan, Michoacán, México. CP. 60250. Tel. 452 5237392

DOI:

https://doi.org/10.29312/remexca.v15i5.3733

Keywords:

Capsicum pubescens, genetic variability

Abstract

The research work was developed at the Plant Genetic Resources Laboratory of the ‘Presidente Juárez’ Faculty of Agrobiology of the Michoacán University of San Nicolás de Hidalgo, in 2019 and 2022. The aim of this research was to evaluate the usefulness of functional markers derived from CYP450 for genetic diversity studies in Capsicum pubescens. The genetic material consisted of 31 cultivated varieties of C. pubescens from three different localities in the state of Michoacán, Mexico. Genomic DNA was obtained based on the protocol of Huang et al. (2013) and two combinations of CYP450 primers were included in the analysis. The amplification products were separated on 8% acrylamide gels and stained with silver nitrate. A total of 85 loci were detected: the CYP2B6F/CYP2C19R combination detected 34 polymorphic loci, while the CYP2C19F/CYP1A1R combination detected only 27. The diversity analysis of C. pubescens identified 1.54 alleles per locus, 1.33 effective number of alleles per locus, Shannon index of 0.3, a heterozygosity index of 0.2 and 60.39% of polymorphic loci. The results obtained show that markers derived from CYP450 are an efficient and low-cost alternative for studies of genetic diversity in plant species.

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Published

2024-08-13

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Escalera-Ordaz, Ana Karen, Héctor Guillén-Andrade, Pedro Antonio López, and Luis Mario Tapia Vargas. 2024. “Genetic Diversity of Capsicum Pubescens by Functional Genomic Markers of CYP450”. Revista Mexicana De Ciencias Agrícolas 15 (5). México, ME:e3733. https://doi.org/10.29312/remexca.v15i5.3733.

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Vol. 15 No. 5 (2024)

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