Identification of bean genotypes resistant to angular leaf spot by molecular markers

Authors

  • Janette Zavala-Páramo Campo Experimental Bajío-INIFAP. Carretera Celaya-San Miguel de Allende km 6.5, Celaya, Guanajuato, México. CP. 38110
  • Jorge Alberto Acosta-Gallegos Campo Experimental Bajío-INIFAP. Carretera Celaya-San Miguel de Allende km 6.5, Celaya, Guanajuato, México. CP. 38110
  • Ana Paulina Rodríguez-Vera Campo Experimental Bajío-INIFAP. Carretera Celaya-San Miguel de Allende km 6.5, Celaya, Guanajuato, México. CP. 38110
  • Brenda Zulema Guerrero-Aguilar Campo Experimental Bajío-INIFAP. Carretera Celaya-San Miguel de Allende km 6.5, Celaya, Guanajuato, México. CP. 38110
  • José Luis Anaya-López Campo Experimental Bajío-INIFAP. Carretera Celaya-San Miguel de Allende km 6.5, Celaya, Guanajuato, México. CP. 38110
  • Víctor Montero-Tavera Campo Experimental Bajío-INIFAP. Carretera Celaya-San Miguel de Allende km 6.5, Celaya, Guanajuato, México. CP. 38110

DOI:

https://doi.org/10.29312/remexca.v15i18.2887

Keywords:

Phaseolus vulgaris, Pseudocercospora griseola, black bean, resistance, SCAR

Abstract

Angular leaf spot caused by Pseudocercospora griseola (Sacc.) Crous & Braun is one of the most common diseases of beans, reducing up to 80% of the harvest if weather conditions are favorable. Genetic improvement for resistance to this disease is one of the most effective strategies. One of the most efficient ways to control this disease is the introduction of genetic resistance mediated by molecular marker-assisted selection. Therefore, the present work aimed to identify genotypes of beans (Phaseolus vulgaris) with resistance to angular leaf spot in Mexican varieties and lines of black, bay, and red beans. To this end, eight specific molecular markers of the SCAR type were used and the correlation between the presence of the markers and the resistance of each genotype measured by inoculation with two strains of the fungus from different physiographic regions under greenhouse conditions was determined. The varieties Negro Comapa, Negro Cotaxtla, Negro Papaloapan, Ouro Negro and the advanced lines of black beans SEN 56, SEN 26, and SCN 7 were resistant to angular leaf spot; in contrast, Bayo Azteca, Bayo INIFAP, the red bean lines SCR 13 and SER 83, as well as the black bean lines SEN 44 and SEN 70 were susceptible. In general, the genotypes with the highest resistance presented the markers CV542014, SAA19, SM02, and SN02. Although the genetic materials were heterogeneous, these molecular markers, considered to be associated with genes with additive effects, could support the selection of P. griseola resistant genotypes and the actions of breeding programs to obtain new resistant varieties.

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References

Beebe, S.; Rengifo, J.; Gaitan, E.; Duque, M. C. and Tohme, J. 2001. Diversity and origin of Andean landraces of common bean. Crop Sci. 41(3):854-862. https://doi.org/10.2135/cropsci2001.413854x.

Beebe, S.; Skroch, P. W.; Tohme, J.; Duque, M. C.; Pedraza, F. and Nienhuis, J. 2000. Structure of genetic diversity among common bean landraces of Mesoamerican origin based on correspondence analysis of RAPD. Crop Sci. 40(1):264-273. https://doi.org/10.2135/cropsci2000.401264x.

Crous, P. W.; Liebenberg, M, M.; Braun, U. and Groenewald, J. Z. 2006. Reevaluating the taxonomic status of Phaeoisariopsis griseola, the causal agent of angular leaf spot of bean. Studies in Mycol. 55(1):163-170. https://doi.org/10.3114/sim.55.1.163.

de Jesús, W. C. J.; Vale, F. X. R.; Coelho, R. R.; Hau, B.; Zambolim, L.; Costa, L. C. and Filho, A. B. 2001. Effects of angular leaf spot and rust on yield loss of Phaseolus vulgaris. Phytopathol. 91(11):1045-1049. https://doi.org/10.1094/PHYTO.2001.91.11.1045.

de Queiroz, V. T.; Sousa, C. S.; Costa, M. R.; Sanglad, D. A.; Arruda, K. M. A.; Souza, T. L. P. O.; Ragagnin, V. A.; Barros, E. G. and Moreira, M. A. 2004. Development of SCAR markers linked to common bean angular leaf spot resistance genes. Ann. Rep. Bean Improv. Coop. 47:237-238.

Doyle, J. J. 1990. Isolation of plant DNA from fresh tissue. Focus. 12:13-15.

Faleiro, F. G.; Ragagnin, V. A.; Schuster, I.; Corrêa, R. X.; Good-God, P. I.; Brommonshenkel, S. H.; Moreira, M. A. and Barros, E. G. 2003. Mapeamento de genes de resistência do feijoeiro à ferrugem, antracnose e mancha-angular usando Marcadores RAPD. Fitopat. Brasil. 28:2-5. http://dx.doi.org/10.1590/S0100-41582003000100009.

Fihlo, A. B.; Carneiro, S. G.; Godoy, C. V.; Amorim, L.; Berger, R. D. and Hau, B. 1997. Angular leaf spot of Phaseolus beans: relationships between disease, healthy leaf area, and yield. Phytopathol. 87(5):506-509. https://doi.org/10.1094/PHYTO.1997.87.5.506.

Gil, J.; Solarte, D.; Lobaton, J. D.; Mayor, V.; Barrera, S.; Jara, C.; Beebe, S. and Raatz, B. 2019. Fine-mapping of angular leaf spot resistance gene Phg-2 in common bean and development of molecular breeding tools. Theor and Applied Genet. 132(7):2003-2016. https://doi.org/10.1007/s00122-019-03334-z.

Gonçalves-Vidigal, M. C.; Cruz, A. S.; Garcia, A.; Kami, J.; Vidigal, F.; Pedro, S.; Sousa, L. L.; McClean, P. P. G. and Pastor-Corrales, M. A. 2011. Linkage mapping of the Phg-1 and Co-14 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar AND 277. Theor and Applied Genet. 122:893-903. Doi: 10.1007/s00122-010-1496-1.

León-Saavedra, I. 2009. La antracnosis y la mancha angular del frijol común (Phaseolus vulgaris L.). Tem. Cienc. Tecnol. 13(39):23-25.

Librelon, S. S.; Souza, E. A. D.; Pereira, R.; Pozza, E. A. and Abreu, A. D. F. B. 2015. Diagrammatic scale to evaluate angular leaf spot severity in primary leaves of common bean. Australas. Plant Pathol. 44(4):385-395. https://doi.org/10.1007/s13313-015-0360-9.

Mahuku, G.; Montoya, C.; Henríquez, M. A.; Jara, C.; Teran, H. and Beebe, S. 2004. Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene. Crop Sci. 44(5):1817-1824. https://doi.org/10.2135/cropsci2004.1817.

Miller, T.; Gepts, P.; Kimno, S.; Arunga, E.; Chilagane, L. A.; Nchimbi-Msolla, S. Namusoke, A.; Namayanja, A. and Tedla, Y. R. 2018. Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database. Afr. J. of Biotechnol. 17(26):818-828. https://doi.org/10.5897/AJB2018.16493.

Montero-Tavera, V.; Escobedo-Landín, M. A.; Acosta-Gallegos, J. A.; Anaya-López, J. L. and Ruiz-Nieto, J. E. 2017. 26S: novel reference genes from leaves and roots of common bean for biotic stress expression studies based on PCR. Legum. Res. 40(3):429-433. Doi: 10.18805/lr.v0iOF.7859.

Nay, M. M.; Mukankusi, C. M.; Studer, B. and Raatz, B. 2019a. Haplotypes at the Phg-2 locus are determining pathotype-specificity of angular leaf spot resistance in common bean. Front. Plant Sci. 10:1126. https://doi.org/10.3389/fpls.2019.01126.

Nay, M. M.; Souza, T. L.; Raatz, B.; Mukankusi, C. M.; Gonçalves‐Vidigal, M. C.; Abreu, A. F. B; Melo, C. L. and Pastor‐Corrales, M. A. 2019b. A review of angular leaf spot resistance in common bean. Crop Sci. 59(4):1376-1391. https://doi.org/10.2135/cropsci2018.09.0596.

Okii, D.; Badji, A.; Odong, T.; Talwana, H.; Tukamuhabwa, P.; Male, A. Mukankusi, C. and Gepts, P. 2019. Recombination fraction and genetic linkage among key disease resistance genes (Co-42/Phg-2 and Co-5/P. ult) in common bean. Afr. J. Biotechnol. 18(29):819-830. https://doi.org/10.5897/AJB2019.16776.

Rodrigues, L. A.; Coelho, G. R.; Valdisser, P. A. M.; Vianello, R. P. and Souza, T. L. P. 2016. Marker-assisted selection integrated to the embrapa common bean breeding program. Embrapa Arroz e Feijão-Artigo em periódico indexado (ALICE). 199-200 pp.

Sietsche, S.; Borém, A.; Carvalho, G. A.; Rocha, R. C.; Paula, T. J.; Barros, E. G. and Moreira, M. A. 2000. RAPD and SCAR markers linked to a gene coffering resistance to angular leaf spot in common bean. J. Phytopathol. 148(2):117-121. Doi: 10.1046/j.1439-0434.2000.00479.x.

SIAP. 2019. Sistema de Información Agroalimentaria y Pesquera. Panorama agroalimentario. Secretaría de Agricultura y Desarrollo Rural. Ciudad de México. 1ra. Ed. 218 p.

Schwartz, H. F.; Correa, F. y Pastor-Corrales, M. 1982. La mancha angular del frijol y su control; guía de estudio. CIAT. Cali, Colombia. 24 p.

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Published

2024-12-15

How to Cite

Zavala-Páramo, Janette, Jorge Alberto Acosta-Gallegos, Ana Paulina Rodríguez-Vera, Brenda Zulema Guerrero-Aguilar, José Luis Anaya-López, and Víctor Montero-Tavera. 2024. “Identification of Bean Genotypes Resistant to Angular Leaf Spot by Molecular Markers”. Revista Mexicana De Ciencias Agrícolas 15 (8). México, ME:e2887. https://doi.org/10.29312/remexca.v15i18.2887.

Issue

Vol. 15 No. 8 (2024)

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Articles

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