Evaluación de tres protocolos para la extracción rápida de ARN total de tejidos de Prosopis juliflora (SW)

Authors

  • Claudia Yared Michel-López , Instituto de Ciencias Agrícolas-Universidad Autónoma de Baja California. Carretera a Delta s/n, ejido Nuevo León, Mexicali, Baja California, México. CP. 21705
  • Daniel González-Mendoza , Instituto de Ciencias Agrícolas-Universidad Autónoma de Baja California. Carretera a Delta s/n, ejido Nuevo León, Mexicali, Baja California, México. CP. 21705
  • Omar Zapata-Pérez , Departamento de Recursos del Mar-CINVESTAV-Unidad Mérida. Antigua carretera a Progreso km 6, Cordemex, Loma Bonita Xcumpich, Mérida, Yucatán, México. CP. 97310
  • Jorge Rubio-Piña , Instituto Tecnológico de Mérida. Av. Tecnológico km 4.5, Mérida, Yucatán, México. CP. 97118
  • Lourdes Cervantes-Díaz , Instituto de Ciencias Agrícolas-Universidad Autónoma de Baja California. Carretera a Delta s/n, ejido Nuevo León, Mexicali, Baja California, México. CP. 21705
  • Manuel de Jesús Bermúdez-Guzmán , Campo Experimental Tecomán-INIFAP. Carretera Colima-Manzanillo km 35, Tecomán, Colima, México

DOI:

https://doi.org/10.29312/remexca.v9i6.788

Keywords:

extraction, mezquites, PCR, RNA

Abstract

The extraction of high quality genomic RNA for PCR amplification, from Prosopis spp., is complicated due to the presence of a high percentage of secondary metabolites that bind or co-precipitate nucleic acids. In the present study, we report a modified protocol of sodium dodecyl sulfate/phenol that includes the elimination of liquid nitrogen in the maceration process, β-mercaptoethanol in the extraction of buffer and step of precipitation of ethanol. The absorbance ratios A260/A280 of the isolated RNA were around 2 to 1.9, suggesting that the DNA fraction was pure and can be used for a subsequent PCR analysis. The RNA isolated by this protocol is of sufficient quality for molecular applications. This technique could be applied to other organisms that have similar substances that make it difficult to extract RNA. Finally, this proposal represents a fast, cheap and effective alternative for the isolation of genomic RNA from fresh leaves of Prosopis spp., even in low-tech laboratories.

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Published

2018-09-24

Issue

Vol. 9 No. 6 (2018)

Section

Articles

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How to Cite

Michel-López, Claudia Yared, Daniel González-Mendoza, Omar Zapata-Pérez, Jorge Rubio-Piña, Lourdes Cervantes-Díaz, and Manuel de Jesús Bermúdez-Guzmán. 2018. “Evaluación De Tres Protocolos Para La extracción rápida De ARN Total De Tejidos De Prosopis Juliflora (SW)”. Revista Mexicana De Ciencias Agrícolas 9 (6): 1259-67. https://doi.org/10.29312/remexca.v9i6.788.

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