Micropropagation and ex-vitro rooting of Beaucarnea hookeri (LEM.) Baker

Authors

  • María del Rosario Chávez-Muñoz Recursos Genéticos y Productividad-Fruticultura-Colegio de Postgraduados. Texcoco, Estado de México. CP. 56230.
  • Gregorio Arellano-Ostoa Recursos Genéticos y Productividad-Fruticultura-Colegio de Postgraduados. Texcoco, Estado de México. CP. 56230. https://orcid.org/0000-0002-9762-9731
  • Yolanda Leticia Fernández Pavía Recursos Genéticos y Productividad-Fruticultura-Colegio de Postgraduados. Texcoco, Estado de México. CP. 56230
  • María de los Ángeles Rodríguez-Elizalde Universidad Autónoma Chapingo-Departamento de Fitotecnia. Texcoco, Estado de México. CP. 56230
  • Eulalia Edith Villavicencio-Gutiérrez Campo Experimental Saltillo-INIFAP. Saltillo, Coahuila. CP. 25315
  • Héctor González Rosas Recursos Genéticos y Productividad-Fruticultura-Colegio de Postgraduados. Texcoco, Estado de México. CP. 56230

DOI:

https://doi.org/10.29312/remexca.v14i7.3223

Keywords:

Calibanus hookeri, endangered, in vitro culture, Nolinaceae, organogenesis

Abstract

Beaucarnea hookeri is a species endemic to Mexico and is listed as threatened in NOM-059-SEMARNAT-2010. In order to micropropagate this species, an experiment was established at the Montecillo Campus, College of Postgraduates in 2022, where the response of the shoots during in vitro multiplication with different concentrations of benzylaminopurine (BAP) and their capacity for ex-vitro rooting during acclimatization were evaluated. An experiment was established with three subsequent subcultures (SC3, SC4, SC4-1); in SC3, shoots generated with 0.5 mg L-1 BAP (SC2) were used; in SC4, healthy shoots generated from SC3; and SC4-1 with shoots from SC3 but with initial hyperhydration (IHH). In SC3, 0.5, 1, 1.5, 2, and 2.5 mg L-1 BAP were evaluated. In SC4, 0 and 0.5 mg L-1 BAP and in SC4-1, concentrations of 0, 0.5, and 1 mg L-1 BAP. During acclimatization, four treatments of Radix® 10 000 (0, 1 000, 2 000, and 4 000 mg kg-1) were evaluated, and shoots from SC2 were used. During multiplication, the number of shoots (NS) was recorded, and SC3 generated 6.35 shoots with the concentration of 1.5 mg L-1 BAP, with the presence of IHH. On average, 9.7 shoots were obtained in SC4, and 5.3 shoots in SC4-1, both subcultures with 0.5 mg L-1 BAP. In addition, there was a recovery of IHH in SC4-1. In rooting, there were no significant differences between treatments, and the control generated 73.3% of shoots with roots (SSR) 80 days after transplantation. Only the shoots of the control treatment in multiplication presented roots, which allowed complete plants to be obtained in 30 days.

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Published

2023-10-31

How to Cite

Chávez-Muñoz, María del Rosario, Gregorio Arellano-Ostoa, Yolanda Leticia Fernández Pavía, María de los Ángeles Rodríguez-Elizalde, Eulalia Edith Villavicencio-Gutiérrez, and Hector González Rosas. 2023. “Micropropagation and Ex-Vitro Rooting of Beaucarnea Hookeri (LEM.) Baker”. Revista Mexicana De Ciencias Agrícolas 14 (7). México, ME:e3223. https://doi.org/10.29312/remexca.v14i7.3223.

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Vol. 14 No. 7 (2023)

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