Effect of electrolyzed water on Pseudomonas syringae pv. Tomato under in vitro conditions

Authors

  • Lilia Mexicano-Santoyo División de estudios de Posgrado e Investigación-Instituto Tecnológico de Roque. Carretera Celaya-Juventino Rosas km 8, Celaya, Guanajuato. AP. 508, CP. 38110
  • Tarsicio Medina-Saavedra Departamento de Ingeniería Agroindustrial-Universidad de Guanajuato. Privada Arteaga s/n, Col. Centro, Salvatierra, Guanajuato. CP. 38900.
  • Mariano Mendoza Elos Instituto Tecnológico de Roque
  • Alejandra Chacón-López División de Posgrado e investigación-Instituto Tecnológico de Tepic. Av. Tecnológico núm. 2595, Col. Lagos del Country, Tepic, Nayarit, México. CP. 63175
  • Adriana Mexicano-Santoyo División de estudios de Posgrado-Instituto Tecnológico de Ciudad Victoria. Boulevard Emilio Portes Gil núm. 1301 Pte. AP. 175, CP. 87010
  • Ulises Miguel López-García División de Posgrado e investigación-Instituto Tecnológico de Tepic. Av. Tecnológico núm. 2595, Col. Lagos del Country, Tepic, Nayarit, México. CP. 63175

DOI:

https://doi.org/10.29312/remexca.v15i7.3175

Keywords:

Acidic water, alkaline water, antimicrobial

Abstract

Pseudomonas syringae pv. Tomato causes bacterial speck in tomato crops, causing stunted growth, late fruit ripening, and yield losses. Pesticides are used to control this phytopathogen, which have been reported to be a source of soil contamination. Because the use of pesticides has increased and, with it, the negative impact, it is necessary to look for efficient and environmentally friendly alternatives. Electrolyzed water is an alternative to the use of pesticides as it has been shown to have an antimicrobial effect. It is produced by electrolysis of dilute solutions of NaCl in an electrolysis cell. The present work evaluated the effect of acidic and alkaline electrolyzed water on PstDC3000 cultures. The experiment was conducted in the agrobiotechnology and electrochemistry laboratory of the Technological Institute of Tepic in 2020. Treatments with acidic and alkaline electrolyzed water were applied for 1, 3, and 6 minutes. The results show total inhibition of PstDC3000 with acidic electrolyzed water treatment at minute 01. On the other hand, treatment with acidic electrolyzed water managed to reduce 26.9%, 42.16%, and 58.53% CFU ml-1 at minutes 1, 3, and 6, respectively. Finally, it was concluded that electrolyzed water affects the growth of PstDC3000 and that pH is a factor that influences its bactericidal activity.

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References

Abhilash, P. C. y Singh, N. 2009. Pesticide use and application: an Indian scenario. Journal of Hazardous Materials. 165(1):1-12. Doi: 10.1016/j.jhazmat.2008.10.061.

Al-Qadiri, H. M.; Al-Holy, M. A.; Shiroodi, S. G.; Ovissipour, M.; Govindan, B. N.; Al-Alami, N. and Rasco, B. 2016. Effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (Venerupis philippinarum) and mussel (Mytilus edulis). International Journal of Food Microbiology. 23:48-53. Doi: 10.1016/j.ijfoodmicro.2016.05.012.

Beuzón, C. R. y Ruiz-Albert, J. 2014. Sistema de secreción tipo III en la interacción de Pseudomona syringae con la planta. Microbiología Molecular. 60(58):130-132.

Cunnac, S.; Lindeberg, M. and Collmer, A. 2009. Pseudomonas syringae type III secretion system effectors: Repertoires in search of functions. Current Opinion in Microbiology. 12(1):53-60. Doi: 10.1016/j.mib.2008.12.003.

Fujiwara, K.; Fujii, T. and Park, J. S. 2009. Comparison of foliar spray efficacy of electrolytically ozonated water and acidic electrolyzed oxidizing water for controlling powdery mildew infection on cucumber leaves. Ozone: Science & Engineering. 31(1):10-14. Doi: 10.1080/01919510802587358.

Gomiero, T. 2018. Food quality assessment in organic vs. conventional agricultural produce: Findings and issues. Applied Soil Ecology. 123(12):714-728. Doi: 10.1016/j.apsoil.2017.10.014.

Hao, J.; Wuyundalai, L. H.; Chen, T.; Zhou, Y.; Su, Y. and Li, L. 2011. Reduction of pesticide residues on fresh vegetables with electrolyzed water treatment. Journal of Food Science. 76(4):C520-C524. Doi: 10.1111/j.1750-3841.2011.02154.x.

Huang, Y R.; Hung, Y. C.; Hsu, S. Y.; Huang, Y. W. and Hwang, D. F. 2008. Application of electrolyzed water in the food industry. Food Control. 19(4):329-345. Doi: 10.1016/j.foodcont.2007.08.012.

Issa-Zacharia, A.; Kamitani, Y.; Tiisekwa, A.; Morita, K. and Iwasaki, K. 2010. In vitro inactivation of Escherichia coli, Staphylococcus aureus and Salmonella spp. using slightly acidic electrolyzed water. Journal of Bioscience and Bioengineering. 110(3):308-313. Doi:10.1016/j.jbiosc.2010.03.012.

Ovissipour, M.; Al-Qadiri, H. M.; Sablani, S. S.; Govindan, B. N.; Al-Alami, N. and Rasco, B. 2015. Efficacy of acidic and alkaline electrolyzed water for inactivating Escherichia coli O104:H4, Listeria monocytogenes, Campylobacter jejuni, Aeromonas hydrophila and Vibrio parahaemolyticus in cell suspensions. Food Control. 53:117-123. Doi: 10.1016/j.foodcont.2015.01.006.

Pangloli, P. and Hung, Y. C. 2011. Efficacy of slightly acidic electrolyzed water in killing or reducing Escherichia coli O157:H7 on iceberg lettuce and tomatoes under simulated food service operation conditions. Journal of Food Science. 76(6):M361-M366. Doi: 10.1111/j.1750-3841.2011.02219.x.

Preston, G. M. 2000. Pseudomonas syringae pv. Tomato: the right pathogen, of the right plant, at the right time. Molecular Plant Pathology. 1(5):263-275.

Rahman, S. M. E.; Khan, I. and Oh, D. H. 2016. Electrolyzed water as a novel sanitizer in the food industry: current trends and future perspective. Compr. Reviews in Food Science and Food Safety. 15(3):471-490.

SAGARPA, 2011. Enfermedades fúngicas y bacterianas del cultivo de tomate en el estado de Nayarit. Folleto técnico núm. 19. 12-66 pp. ISBN: 978-607-425-720-5.

Sanaullah, M.; Usman, M.; Wakeel, A.; Cheema, S. A.; Ashraf, I. and Farooq, M. 2020. Terrestrial ecosystem functioning affected by agricultural management systems: a review. Soil and Tillage Research. 196:1-11. Doi:10.1016/j.still.2019.104464.

Shiroodi, S. G. and Ovissipour, M. 2018. Electrolyzed water application in fresh produce sanitation. Postharvest Disinfection of Fruits and Vegetables. 67-89 pp. Doi: 10.1016/b978-0-12-812698-1.00003-0.

Sun, J. L.; Zhang, S. K.; Chen, J. Y.; and Han, B. Z. 2012. Efficacy of acidic and basic electrolyzed water in eradicating staphylococcus aureus biofilm. Canadian Journal of Microbiology. 58(4):448-454. Doi: 10.1139/w2012-005.

Xin, X. F. and He, S. Y. 2013. Pseudomonas syringae pv. Tomato DC3000: a model pathogen for probing disease susceptibility and hormone signaling in plants. Annual Review of Phytopathology. 51:473-498. Doi: 10.1146/annurev-phyto-082712-102321.

Zhao, Y.; Thilmony, R.; Bender, C. L.; Schaller, A.; He, S. Y. and Howe, G. A. 2003. Virulence systems of Pseudomonas syringae pv. Tomato promote bacterial speck disease in tomato by targeting the jasmonate signaling pathway. The Plant Journal. 36(4):485-499. Doi:10.1046/j.1365-313X.2003.01895.x.

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Published

2024-12-12

How to Cite

Mexicano-Santoyo, Lilia, Tarsicio Medina-Saavedra, Mariano Mendoza Elos, Alejandra Chacón-López, Adriana Mexicano-Santoyo, and Ulises Miguel López-García. 2024. “Effect of Electrolyzed Water on Pseudomonas Syringae Pv. Tomato under in Vitro Conditions”. Revista Mexicana De Ciencias Agrícolas 15 (7). México, ME:e3175. https://doi.org/10.29312/remexca.v15i7.3175.

Issue

Vol. 15 No. 7 (2024)

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