Dynamics of in vitro growth of Phytophthora cinnamomic in alternative culture media

Authors

  • Anselmo Hernández Pérez Departamento de Parasitología-Universidad Autónoma Agraria Antonio Narro. Buenavista, Saltillo, Coahuila, México. CP. 25315
  • Yisa María Ochoa Fuentes Departamento de Parasitología-Universidad Autónoma Agraria Antonio Narro. Buenavista, Saltillo, Coahuila, México. CP. 25315
  • Ernesto Cerna Chávez CONACYT-Departamento de Parasitología-Universidad Autónoma Agraria Antonio Narro. Buenavista, Saltillo, Coahuila, México. CP. 25315
  • Juan Carlos Delgado Ortiz Departamento de Parasitología-Universidad Autónoma Agraria Antonio Narro. Buenavista, Saltillo, Coahuila, México. CP. 25315
  • Mariana Beltrán Beach CULTA SA de CV. Bulevar Luis Echeverría Álvarez núm. 1700, Colonia Altavista, CD. Mante, Tamaulipas, México. CP. 89880
  • Omegar Hernández Bautista CULTA SA de CV. Bulevar Luis Echeverría Álvarez núm. 1700, Colonia Altavista, CD. Mante, Tamaulipas, México. CP. 89880
  • Luis Mario Tapia-Vargas Campo Experimental Uruapan-INIFAP. Avenida. Latinoamericana núm. 1101, Col. Revolución, Uruapan, Michoacán, México. CP. 60500.

DOI:

https://doi.org/10.29312/remexca.v0i23.2032

Keywords:

avocado, culture media, growth rate

Abstract

Mexico is the leading producer, consumer and exporter of avocado in the world. The study of soil macrobiotics becomes more important in the avocado wilt complex because in these pathology biotic and abiotic causative agents can be implicated, being the Oomycete Phytophthora cinnamomi Rands the main radical disease of avocado cultivation. The objective of the present investigation was to evaluate the dynamics of the in vitro growth of Phytophthora cinnamomi in alternative culture media. In the months of October and November 2016, root samples were collected from avocado trees (Persea americana Mill. Var. Hass) in the INIFAP experimental orchard located in San Juan Nuevo Parangaricutiro, Michoacán. The isolates were identified morphologically and molecularly. The measurements of the growth diameters of each strain were recorded in vitro according to time. Linear regression was carried out using generalized linear models (GLM) to obtain the parameters of intercept and slope, significance of the regression and the R2 value adjusted. The analyzes were carried out using the statistical program R 3.4. According to the results obtained, the culture medium Rye-agar was the most efficient for the proliferation of P. cinnamomi.

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Published

2019-09-17

How to Cite

Hernández Pérez, Anselmo, Yisa María Ochoa Fuentes, Ernesto Cerna Chávez, Juan Carlos Delgado Ortiz, Mariana Beltrán Beach, Omegar Hernández Bautista, and Luis Mario Tapia-Vargas. 2019. “Dynamics of in Vitro Growth of Phytophthora Cinnamomic in Alternative Culture Media”. Revista Mexicana De Ciencias Agrícolas, no. 23 (September). México, ME:331-38. https://doi.org/10.29312/remexca.v0i23.2032.

Issue

No. 23 (2019): Especial

Section

Investigation notes

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