In vitro germination of biznaga cabuchera
DOI:
https://doi.org/10.29312/remexca.v9i3.1226Keywords:
Ferocactus pilosus, H2O2, H2SO4, germination time, growth regulator, standard germinationAbstract
Ferocactus pilosus [(Galeotti ex Salm-Dyck) Werdermann] is a cactacea known as biznaga, which shows slow growth and is listed as a species at risk of extinction. The objective of the present study was to carry out an in vitro test in order to study the effect of chemical treatments and growth regulators, as promoters of seed germination collected in two consecutive years. The chemical products studied were H2SO4 and H2O2 and as the AG3, AIA and ANA phytoregulators, under different immersion and concentration times, respectively. The allocation of treatments was under a completely randomized design. For 30 days, the number of seeds germinated every 2 days was recorded and the standard germination was determined. Logistic regression was used to study the effect of the study factors on the proportion of germinated seeds. The standard germination of the biznaga is 82% with the H2SO4 treatment, while the germination of the seed starts between 2 and 6 days after sowing in both years and the representative logarithmic phase is between 8 and 20 days with H2SO4 in 2015. The biznaga seeds sampled in 2016 show greater slowness to cover the initial phase of germination, covered in 16 days. The application of H2SO4 shortened the germination time by 4 days and increased the standard germination by 82%, while the H2O kept the standard germination constant (±50%) and the germination response time was found between 6 and 10 days after the germination, planting in vitro.
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