Antioxidant activity and composition of Clinopodium tomentosum in wet and dry extraction

Authors

  • Miguel Angel Enríquez-Estrella Escuela de Ingeniería Agroindustrial-Facultad de Ciencias de la Tierra-Universidad Estatal Amazónica. Puyo, Ecuador
  • Gabriela Joseth Serrano-Torres Facultad de Ingeniería-Universidad Nacional de Chimborazo. Riobamba, Ecuador
  • Silvia Hipatia Torres-Rodriguez Facultad de Ingeniería-Universidad Nacional de Chimborazo. Riobamba, Ecuador
  • Dennis Gabriel Cuadrado-Ayala Consultor independiente

DOI:

https://doi.org/10.29312/remexca.v16i3.3630

Keywords:

active principle, bioactive, extracts, phytochemicals

Abstract

In ancient times, various plant species were used in traditional medicine due to their properties, being utilized in diverse processes, both in the food and pharmaceutical sectors. The purpose of this study was to characterize the bioactive components, specifically antioxidants and polyphenols, present in Clinopodium tomentosum in both dry and wet samples. An ultrasound-assisted extraction method was implemented to obtain the active compounds. Subsequently, the identification and quantification of the extracted compounds were performed using the FRAP (Ferric Reducing Antioxidant Power) and Folin-Ciocalteu assays. As a result of this process, it was observed that extraction from dry matter yields higher concentrations, with 1.49 mg L-1 of polyphenols and 1.27 mg L-1 of antioxidants, compared to wet extraction, which yielded concentrations of 1.39 mg L-1 of polyphenols and 1.04 mg L-1 of antioxidants. The characterization of Clinopodium tomentosum enabled the identification of its bioactive components, paving the way for future research on the identification of its phytochemical components and their potential application in both food and non-food industries.

Downloads

Download data is not yet available.

References

Antolovich, M. A. and Crozier, A. M. 2000. Sample preparation in the analysis of antioxidants. Analyst. 125(5):989-1009. Doi.org/10.1039/b006426j.

Benzo, M.; Gilardoni, G.; Gandini, C.; Caccialanza, G.; Finzi, P. V.; Vidari, G. and Layedra, P. 2007. Determination of the threshold odor concentration of main odorants in essential oils using gas chromatography olfactometry incremental dilution technique. Journal of Chromatography A. 1150(1-2):131-135. Doi.org/10.1016/j.chroma.2006.11.008.

Boots, A. W.; Haenen, G. R. and Bast, A. B. 2008. Health effects of quercetin: from antioxidant to nutraceutical. European Journal of Pharmacology. 585(2-3):325-337. Doi.org/10.1016/j.ejphar.2008.03.008.

Drew, B. T. and Sytsma, K. J. 2012. Phylogenetics, biogeography and staminal evolution in the tribe Mentheae (Lamiaceae). American Journal of Botany. 99(5):933-953.

Duthie, S. J.; Ma, A. B.; Ross, M. A. and Collins, A. R. 1996. Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer Research. 56(6):1291-1295.

Ebert, A. W. and Engels, J. M. 2020. Plant biodiversity and genetic resources matter! Plants. 9(12):1706.04-06.

Enríquez-Estrella, M. 2021. Evaluation of the antioxidant capacity and phenolic content of the essential oil of dry and wet leaves of guaviduca (Piper carpunya Ruiz & Pav.). Semiárida. 31(1):09-15. Doi.org/10.19137/semiarida.2021(01).09-15.

Enriquez-Estrella, M. A.; Arboleda, L. F.; Salous, A. K. and Torres, S. H. 2023. Biological activity of lemongrass (Cymbopogon citratus) and its applications in the industry. Journal of Science and Technology. 40(1):90-97. Doi.org/10.36995/j.recyt.2023.40.010.

Estrella, J. 2015. Biodiversity and genetic resources: a guide for their use and access in Ecuador, Quito. EcoCiencia.

Fitzgerald, M. D.; Heinrich, M. A. and Booker, A. E. 2020. Medicinal plant analysis: A historical and regional discussion of emergent complex techniques. Frontiers in Pharmacology. 10(1):3-5.

Gachet, M. S.; Lecaro, J. S.; Kaiser, M. R.; Brun, R. A.; Navarrete, H. A.; Munoz, R. A. and Schühly, W. A. 2010. Assessment of anti-protozoal activity of plants traditionally used in Ecuador in the treatment of leishmaniasis. Journal of Ethnopharmacology. 128(1):184-197.

González-Rodríguez, R. and Cardentey-García, J. 2016. Knowledge about natural and traditional medicine by residents of general integral medicine. Revista Medicina Electrónica. 38(5):689-696. http://scielo.sld.cu/scielo.php?script=sci-arttext&pid=S168418242016000500004&lng=en.

Jarić, S.; Kostić, O.; Mataruga, Z.; Pavlović, D.; Pavlović, M.; Mitrović, M. and Pavle, P. 2018. Science Direct. Retrieved from https://www.sciencedirect.com/science/article/abs/pii/S0378874117321852#preview-section-introduction.

Justicia, R. M. 2007. Ecuador's chocó andean corridor: a landscape approach for conservation and sustainable development (University of Georgia). http://getd.libs.uga.edu/pdfs/justicia-rebeca-m-200712-phd.pdf.

McGaw, L. J.; Jäger, A. C.; Grace, O. O.; Fennel, C. A. and Staden, J. V. 2005. Medicinal plants. In ethics in agricultura an African perspective. Dordrecht. Springer Netherlands. 67-83 pp.

Naczk, M. and Shahidi, F. 2006. Phenolics in cereals, fruits, and vegetables: Occurrence, extraction, and analysis. Journal of Pharmaceutical and Biomedical Analysis. 41(5):1523-1542.

Pulsan, A.; García-Collado, M.; Fernández-Ortega, M. and Torres-Quiala, M. 2015. Phytotherapy and apitherapy in the work of José Martí. Revista Información Científica. 92(4):945-955. http://www.revinfcientifica.sld.cu/index.php/ric/article/view/212/1384.

Saltos, M. B. V.; Puente, B. F. N.; Malafronte, N. A. and Braca, A. B. 2014. Phenolic compounds from Clinopodium tomentosum (Kunth) Govaerts (Lamiaceae). Journal of the Brazilian Chemical Society. 25(11):2121-2124.

Teng, H. K.; Ghafoor, K. H. and Choi, Y. H. 2009. Optimization of microwave assisted extraction of active components from Chinese quince using response surface methodology. Journal of the Korean Society for Applied Biological Chemistry. 52(1):694-701. https://doi.org/10.3839/jksabc.2009.114.

Turkmen, N. A.; Sari, F. A. and Velioglu, Y. S. 2006. Effects of extraction solvents on concentration and antioxidant activity of black and green tea polyphenols determined by ferrous tartrate and Folin Ciocalteu methods. Food Chemistry. 99(4):835-841. https://doi.org/10.1016/j.foodchem.2005.08.034.

Zambrano, L.; Buena, M.; Mancera, J. and Jiménez, E. 2015. Ethnobotanical study of medicinal plants used by the inhabitants of the rural area of Parroquia San Carlos, Quevedo, Ecuador. Universidad y Salud. 17(9):97-111.

Zielińska, S. C. and Matkowski, A. B. 2014. Phytochemistry and bioactivity of aromatic and medicinal plants from the genus Agastache (Lamiaceae). Phytochemistry Reviews. 13(3):391-416.

Published

2025-04-24

How to Cite

Enríquez-Estrella, Miguel Angel, Gabriela Joseth Serrano-Torres, Silvia Hipatia Torres-Rodriguez, and Dennis Gabriel Cuadrado-Ayala. 2025. “Antioxidant Activity and Composition of Clinopodium Tomentosum in Wet and Dry Extraction”. Revista Mexicana De Ciencias Agrícolas 16 (3). México, ME:e3630. https://doi.org/10.29312/remexca.v16i3.3630.

Issue

Section

Articles

Most read articles by the same author(s)