Lupinus response to chemical scarification and two culture media in in vitro propagation
DOI:
https://doi.org/10.29312/remexca.v16i2.3437Keywords:
dormancy, fabaceae, scarification, tissue cultureAbstract
In Mexico, the genus Lupinus has a great richness of species and its seeds generally show dormancy. The objective was to evaluate the effect of chemical scarification in two culture media on seeds of three species of Lupinus. The research was conducted in the tissue culture laboratory of the Tabasco Campus of the College of Postgraduates. The following was used: three times (20, 30 and 40 min) of immersion in 98% sulfuric acid, seeds of three species of Lupinus, L. campestris, L. exaltatus, and L. montanus, and MS and Gamborg culture media with and without Fe-EDTA. The variables evaluated were germination percentage, root length, stem length, and number of leaves. The results showed that there was a significant interaction between the treatments evaluated, the immersion time in sulfuric acid influenced the germination of the seeds. The highest germination percentage (83) was achieved in a 30 min immersion for L. exaltatus. In contrast, the germination percentage was 63 for L. campestris, and 12 for L. montanus. In the Gamborg culture medium with Fe-EDTA, the germination percentage was 82 and without Fe-EDTA, it was 72 for L. exaltatus, followed by L. campestris and L. montanus. The growth variables evaluated showed high values in the Gamborg culture medium without the addition of Fe-EDTA; in the opposite case, the variables analyzed showed increases in the values obtained. In the MS culture medium with and without Fe-EDTA, the values found were lower compared to the Gamborg culture medium.
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