Effect of plant growth-promoting rhizobacteria on agronomic and macronutrient parameters in pak choi
DOI:
https://doi.org/10.29312/remexca.v14i4.3118Keywords:
Rhizobacteria, Pak choi, Pseudomonas tolaasii, Arthrobacter pokkaliiAbstract
Pak choi is a vegetable of high nutritional value and palatability, which has promoted the increase of its cultivation and consumption outside Asia, its region of origin. This work evaluated the effect of the inoculation of three strains of plant growth-promoting rhizobacteria (PGPR) Arthrobacter pokkalii JLB4, Pseudomonas tolaasii P61 and Pseudomonas tolaasii A46 on pak choi plants under controlled conditions in a high tunnel in 2021. Pak choi seedlings without inoculation were used as a control and two inoculations were performed, the first immediately after transplantation of the seedlings into bags with 5 kg of soil and the second one month later. The plants were simultaneously fertilized with inoculation with NPK in a ratio 164-53-187. The results obtained showed that the strain Pseudomonas tolaasii P61 was the one that had the greatest effect on dry weight (23.04 ±0.057 g plant-1), as well as on the content of P (0.1704 ±0.001 g plant-1) and S (0.1847 ±0.004 g plant-1) compared to the control, with increases of 11.5%, 10.8% and 12.3%, respectively. The strain Arthrobacter pokkalii JLB4 caused greater root growth, the root volume (75 ±5 ml) was 49% higher than the control. However, it had no effect on the dry weight of the aerial part. The results suggest that the combined use of fertilizer and PGPR leads to greater accumulation of biomass and nutrients, such as P, K and S, than the application of fertilizer alone.
Downloads
References
Alcántar, G. G. y Sandoval, V. M. 1999. Manual de análisis químico de tejido vegetal. Guía de muestreo, preparación, análisis e interpretación. Publicación especial núm. 10 de la Sociedad Mexicana de la Ciencia del Suelo AC. Chapingo, Estado de México. 156 p. Alexander, A.; Mishra, A. and Jha, B. 2019. Halotolerant rhizobacteria: a promising probiotic for saline soil-based agriculture. In saline soil-based agriculture by halotolerant microorganisms. Springer, Singapore. 53-73 pp. https://doi.org/10.1007/978-981-13-8335-9-3.
Almeida, H. J. D.; Carmona, V. M. V.; Cavalcante, V. S.; Prado, R. D. M.; Flores, R. A.; Borges, B. M. M. N. and Mauad, M. 2020. Nutritional and visual diagnosis in broccoli (Brassica oleracea var. Italica L.) Plants: disorders in physiological activity, nutritional efficiency, and metabolism of carbohydrates. Agronomy. 10(10):1572-1579. https://doi.org/10.3390/ agronomy10101572. Basu, A.; Prasad, P.; Das, S. N.; Kalam, S.; Sayyed, R. Z.; Reddy, M. S. and El Enshasy, H. 2021. Plant growth promoting rhizobacteria (PGPR) as green bioinoculants: recent developments, constraints, and prospects. Sustainability. 13(3):1140-1148. https://doi.org/10.3390/ su13031140.
Blažević, I.; Montaut, S.; Burčul, F.; Olsen, C. E.; Burow, M.; Rollin, P. and Agerbirk, N. 2020. Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants. Phytochemistry. 169:112100. https://doi.org/10.1016/ j.phytochem.2019.112100.
Castagno, L. N.; Sannazzaro, A. I.; Gonzalez, M. E.; Pieckenstain, F. L. and Estrella, M. J. 2021. Phosphobacteria as key actors to overcome phosphorus deficiency in plants. Ann. Appl. Biology. 178(2):256-267. https://doi.org/10.1111/aab.12673.
Gomes, D. G.; Radi, A. J. and De Aquino, G. S. 2018. Growth-promoting bacteria change the development of aerial part and root system of canola. Semina: Ciênc. Agrár. 39(6):2375-2384. https://doi.org/10.5433/1679-0359.2018v39n6p2375.
Grover, M.; Bodhankar, S.; Sharma, A.; Sharma, P.; Singh, J. and Nain, L. 2020. PGPR mediated alterations in root traits: way towards sustainable crop production. Front. Sustainable Food Syst. 4:287-295. https://doi.org/10.3389/fsufs.2020.618230.
Ishida, M.; Hara, M.; Fukino, N.; Kakizaki, T. and Morimitsu, Y. 2014. Glucosinolate metabolism, functionality, and breeding for the improvement of Brassicaceae vegetables. Breed. Sci. 64(1):48-59. https://doi.org/10.1270/jsbbs.64.48. DOI: https://doi.org/10.1270/jsbbs.64.48
Johnson, T. L.; Dinkova-Kostova, A. T. and Fahey, J. W. 2016. Glucosinolates from the Brassica vegetables and their health effects. In encyclopedia of food and health. Elsevier Inc. 248-255 pp. https://doi.org/10.1016/B978-0-12-384947-2.00354-8. DOI: https://doi.org/10.1016/B978-0-12-384947-2.00354-8
Krishnan, R.; Menon, R. R.; Tanaka, N.; Busse, H. J.; Krishnamurthi, S. and Rameshkumar, N. 2016. Arthrobacter pokkalii sp. nov, a novel plant associated actinobacterium with plant beneficial properties, isolated from saline tolerant pokkali rice, Kerala, India. PLoS One. 11(3):e0150322. https://doi.org/10.1371/journal.pone.0150322. DOI: https://doi.org/10.1371/journal.pone.0150322
Leontidou, K.; Genitsaris, S.; Papadopoulou, A.; Kamou, N.; Bosmali, I.; Matsi, T.; Madesis, P.; Vokou, D.; Karamanoli, K. and Mellidou, I. 2020. Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterization and exploration of Phyto-beneficial traits. Sci. Rep. 10:14857. https://doi.org/10.1038/s41598-020-71652-0.
Ljung K. 2013. Auxin metabolism and homeostasis during plant development. Development. 140(5): 943-950. https://doi.org/10.1242/dev.086363. DOI: https://doi.org/10.1242/dev.086363
Lobo, C. B.; Tomás, M. S. J.; Viruel, E.; Ferrero, M. A. and Lucca, M. E. 2019. Development of low-cost formulations of plant growth-promoting bacteria to be used as inoculants in beneficial agricultural technologies. Microbiol. Res. 219:12-25. https://doi.org/10. 1016/j.micres.2018.10.012.
Malik, D. K. and Sindhu, S. S. 2011. Production of indole acetic acid by Pseudomonas sp.: effect of coinoculation with Mesorhizobium sp. cicer on nodulation and plant growth of chickpea (Cicer arietinum). Physiol. Mol. Biol. Plants. 17(1):25-32. https://doi.org/10.1007/s12298-010-0041-7. DOI: https://doi.org/10.1007/s12298-010-0041-7
Marschner, P.; Solaiman, Z. and Rengel, Z. 2007. Brassica genotypes differ in growth, phosphorus uptake and rhizosphere properties under P-limiting conditions. Soil. Biol. Biochem. 39(1):87-98. https://doi.org/10.1016/j.soilbio.2006.06.014. DOI: https://doi.org/10.1016/j.soilbio.2006.06.014
Maseko, I.; Beletse, Y. G.; Nogemane, N.; du Plooy, C. P.; Musimwa, T. R. and Mabhaudhi, T. 2017. Productivity of non-heading Chinese cabbage (Brassica rapa subsp. chinensis) under different agronomic management factors, S. Afr. J. Plant Soil. 34(4):275-282. https://doi.org/10.1080/02571862.2017.1295324. DOI: https://doi.org/10.1080/02571862.2017.1295324
Qing, C. M.; Fan, L.; Lei, Y.; Bouchez, D.; Tourneur, C.; Yan, L. and Robaglia, C. 2000. Transformation of Pakchoi (Brassica rapa L. ssp. chinensis) by agrobacterium infiltration. Mol. Breed. 6:67-72. https://doi.org/10.1023/A:1009658128964. DOI: https://doi.org/10.1023/A:1009658128964
Tsukanova, K. A.; Meyer, J. J. M. and Bibikova, T. N. 2017. Effect of plant growth promoting Rhizobacteria on plant hormone homeostasis. S. Afr. J. Bot. 113:91-102. https://doi.org/10.1016/j.sajb.2017.07.007. DOI: https://doi.org/10.1016/j.sajb.2017.07.007
Vanneste, S. y Friml, J. 2009. Auxin: a trigger for change in plant development. Cell. 136:1005-1016. https://doi.org/10.1016/j.cell.2009.03.001. DOI: https://doi.org/10.1016/j.cell.2009.03.001
Vega-Celedón, P.; Canchignia-Martínez, H.; González, M. y Seeger, M. 2016. Biosíntesis de ácido indol-3-acético y promoción del crecimiento de plantas por bacterias. Cultivos Tropicales. 37:33-39.
Wang, J.; Li, R.; Zhang, H.; Wei, G. and Li, Z. 2020. Beneficial bacteria activate nutrients and promote wheat growth under conditions of reduced fertilizer application. BMC Microbiol. 20(1):1-12. https://doi.org/10.1186/s12866-020-1708-z.
Xu, X.; Du, X.; Wang, F.; Sha, J.; Chen, Q.; Tian, G. and Jiang, Y. 2020. Effects of potassium levels on plant growth, accumulation and distribution of carbon, and nitrate metabolism in apple dwarf rootstock seedlings. Front Plant Sci. 11:904. https://doi.org/10.3389/ fpls.2020.00904.
Zang, Y.; Zhang, H.; Huang, L.; Wang, F.; Gao, F.; Lv, X. y Zhu, Z. 2015. Glucosinolate enhancement in leaves and roots of pak choi (Brassica rapa ssp. chinensis) by methyl jasmonate. Hortic. Environ Biotechnol. 56(6):830-840. https://doi.org/10.1007/s13580-015-0079-0. DOI: https://doi.org/10.1007/s13580-015-0079-0
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Revista Mexicana de Ciencias Agrícolas
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The authors who publish in Revista Mexicana de Ciencias Agrícolas accept the following conditions:
In accordance with copyright laws, Revista Mexicana de Ciencias Agrícolas recognizes and respects the authors’ moral right and ownership of property rights which will be transferred to the journal for dissemination in open access. Invariably, all the authors have to sign a letter of transfer of property rights and of originality of the article to Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) [National Institute of Forestry, Agricultural and Livestock Research]. The author(s) must pay a fee for the reception of articles before proceeding to editorial review.
All the texts published by Revista Mexicana de Ciencias Agrícolas —with no exception— are distributed under a Creative Commons License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned.
The author(s) can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Revista Mexicana de Ciencias Agrícolas (for example include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Revista Mexicana de Ciencias Agrícolas.
For all the above, the authors shall send the Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a PDF file to: revista_atm@yahoo.com.mx; cienciasagricola@inifap.gob.mx; remexca2017@gmail.
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 International license.
