Identifying hybrids of Citrus aurantifolia×Citrus limon using simple sequence repeats (SSR) markers
DOI:
https://doi.org/10.29312/remexca.v8i6.309Keywords:
apomixis, citrus, microsatellites, molecular markersAbstract
Apomixis is a type of asexual reproduction where the seeds formation carries genetically identical embryos to the parent, constituting an obstacle in breeding programs for many plant species, including citrus. The identification of hybrid plants is carried out by morphological characters, isoenzymatic assays and molecular markers. The latter have been used more frequently because of their accuracy, standind out the use of random amplified polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR). In Mexican lime (C. aurantifolia) RAPD markers have only been used for hybrid identification, so there are no reports that use SSR markers for this purpose. The aim of this paper was to identify hybrids derived from controlled pollination between C. aurantifolia var. “Colimex”×C. limon var. “Rosenberg” and its reciprocal using SSR molecular markers. During the 2014-2016 years, leaves of lime trees of approximately 12 months of age were collected, which are established in the Campo Experimental Tecomán of the INIFAP. A total of eight SSR molecular markers were evaluated on the progenitors used in this study and the TAA45 and cAGG09 oligonucleotides were selected for the hybrids identification in the two progeny populations. From a total of 40 and 43 F1 individuals from the bi-directional cross between “Colimex”דRosenberg”, 17 and 35 hybrid plants were identified, respectively. The results indicate that SSR markers are efficient and reliable for the identification of Mexican lime hybrids.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
The authors who publish in Revista Mexicana de Ciencias Agrícolas accept the following conditions:
In accordance with copyright laws, Revista Mexicana de Ciencias Agrícolas recognizes and respects the authors’ moral right and ownership of property rights which will be transferred to the journal for dissemination in open access. Invariably, all the authors have to sign a letter of transfer of property rights and of originality of the article to Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) [National Institute of Forestry, Agricultural and Livestock Research]. The author(s) must pay a fee for the reception of articles before proceeding to editorial review.
All the texts published by Revista Mexicana de Ciencias Agrícolas —with no exception— are distributed under a Creative Commons License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned.
The author(s) can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Revista Mexicana de Ciencias Agrícolas (for example include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Revista Mexicana de Ciencias Agrícolas.
For all the above, the authors shall send the Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a PDF file to: revista_atm@yahoo.com.mx; cienciasagricola@inifap.gob.mx; remexca2017@gmail.
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 International license.
