Presence of Varroa destructor, Nosema spp. and Acarapis woodi in colonies of bees from Tabasco, Mexico

Authors

  • Jesús Froylán Martínez Puc National Technology of Mexico-IT China. Street 11 s/n x 22 and 28, China, Campeche. CP. 24520
  • Juan Florencio Gómez Leyva National Technology of Mexico-IT Tlajomulco. Road to San Miguel Cuyutlán km 10, Tlajomulco de Zúñiga, Jalisco. CP. 45640
  • Nicolás González Cortés Rivers Multidisciplinary Academic Division-Juarez Autonomous University of Tabasco. Tenosique-Estapilla highway km 1, Cocoyol, Tenosique de Pino Suárez, Tabasco. CP. 86900
  • Francisco Javier Catzím Rojas National Technology of Mexico-IT Olmec Zone. Ignacio Zaragoza s/n, Villa Ocuiltzapotlán, Downtown, Tabasco. CP. 86250
  • Yessenia Sánchez Melo National Technology of Mexico-IT Olmec Zone. Ignacio Zaragoza s/n, Villa Ocuiltzapotlán, Downtown, Tabasco. CP. 86250
  • Emeterio Payró de la Cruz National Technology of Mexico-IT Olmec Zone. Ignacio Zaragoza s/n, Villa Ocuiltzapotlán, Downtown, Tabasco. CP. 86250

DOI:

https://doi.org/10.29312/remexca.v13i2.3033

Keywords:

Apis mellifera, Acarapis woodi, Nosema spp., Varroa destructor

Abstract

Bee (Apis mellifera) diseases cause great economic losses and are considered the main cause of the high loss of colonies. In this study, the frequency and infestation levels of Varroa destructor and Acarapis woodi were determined. Likewise, the frequency and infection levels of Nosema spp. in colonies of honeybees from Tabasco, Mexico, were determined. During October to November 2020, bee samples were collected in 112 colonies in the five subregions of Tabasco. The frequency and levels of infestation of V. destructor were diagnosed using the capped brood comb fragment technique, as well as the washing of adult bees. The quantification of spores of Nosema spp. was performed by macerating abdomens and visual identification of A. woodi in tracheas using a stereoscopic microscope. At the state level, it was found a frequency of V. destructor in capped brood (FVCO)= 69.64%; level of infestation of V. destructor in capped brood (NIVCO)= 13.86% (p= 0.419), likewise, it was observed a frequency of V. destructor in adult bees (FVAA)= 92.86%; level of infestation of V. destructor in adult bees (NIVAA)= 3.88% (p= 0.944). It was observed a frequency of Nosema spp. (FN)= 91.96%; infection level of Nosema spp. (NIN)= 133 738 ±156 221 spores/bee. All samples were negative for A. woodi. The results indicate that the presence of V. destructor and Nosema spp. are more frequent in the subregions studied, the levels of infestation of V. destructor and the levels of infection of Nosema spp. are low. However, extreme values that exceed the tolerable limits were found in various communities, which could represent foci of attention before possible dissemination of these pathogens.

Downloads

Download data is not yet available.

References

Acosta-Muñiz, C. H.; Romo, Ch. A. y Ríos, V. C. 2014. Detección de nosemosis en alzas de miel en colonias del estado de Chihuahua. Fundación Produce Chihuahua AC. http://siproduce.sifupro.org.mx/seguimiento/archivero/8/2013/anuales/anu-886-6-2014-05-5.pdf.

Bailey, L. 1983. Patología de las abejas. (Ed.). Acribia. Zaragoza, España. 150 p.

Bravo, J.; Carbonell, V.; Valdebenito, J. T.; Figueroa, C.; Valdovino, C. E.; Martín-Hernández, R.; Higes, M. and Delporte, C. 2014. Identification of Nosema ceranae in the Valparaiso District, Chile. Archivos de Medicina Veterinaria. 46:487-491.

Buchmann, S. and Nabhan, G. 1996. The forgotten pollinators. Island Press, Washington, 1032 DC.

Cantwell, G. E. 1970. Standard methods for counting nosema spores. Am. Bee J. 110(6):222-223.

Casavantes, P. F. 2011. PCR identification of Nosema apis and Nosema ceranae in honeybees’ colonies from beekeepers of Chihuahua. Center for Food Research and Development, Cuauhtémoc, Chihuahua, Mexico City.

Coelho, F.; Santos, J. and Bliman, P. 2015. Behavioral modulation of the coexistence between Apis mellifera and Varroa destructor: a defense against colony collapse? Peer J. PrePrints. 17(3):39-44.

De Jong, D.; Roma, D. A. and Goncalves, L. S. 1982. A comparative analysis of shaking solutions for the detection of Varroa jacobsoni on adult honeybees. Apidologie. 13:297-306.

DOF. 1994. Diario Oficial de la Federación. NOM-001-ZOO-1994. Norma oficial mexicana, campaña nacional contra la varroasis de las abejas. http://www.dof.gob.mx/nota-detalle.php?codigo=4691340&fecha=28/04/1994.

FAO. 2021. Food and Agriculture Organization of the United Nations. Sustainable development goals. https://www.un.org/sustainabledevelopment/es/.

FAO-FAOSTAT. 2021. Food and Agriculture Organization of the United Nations, Statistics Division. Natural honey production: top 10 average producers 1993-2019. http://www.fao.org/faostat/es/#data/QL/visualize.

Fries, I.; Chauzat, M. P.; Chen, Y. P.; Doublet, V.; Genersch, E.; Gisder, S.; Higes, M.; McMahon, D. P.; Martín-Hernández, R.; Natsopoulou, M.; Paxton, R. J.; Tanner, G.; Webster, T. C. and Williams, G. R. 2013. Standard methods for Nosema research. J. Apicultural Res. 52(1)12-17.

García, F. C. y Arechavaleta, V. M. E. 2018. Prevalencia de la acariosis traqueal y niveles de infestación de Acarapis woodi en colonias de abejas de Morelos, México. Rev. Mex. Cienc. Pec. 9(3)567-575.

Guerrero, M. C.; Correa-Benítez, A.; Hamiduzzaman, M. and Guzman-Novoa, E. 2016. Nosema ceranae is an old resident of honeybee (Apis mellifera) colonies in Mexico, causing infection levels of one million spores per bee or higher during summer and fall. J. Invertebrate Pathol. 141:38-40.

Guzmán, E.; Hamiduzzaman, M.; Arechavaleta, V. M. E.; Koleoglu, G.; Valizadeh, P. and Correa, B. A. 2011. Nosema ceranae has parasitized Africanized honeybees in Mexico since at least 2004. J. Apicultural Res. 50(2):167-169.

Hinojosa, A. y González, D. 2004. Frecuencia de parásitos en Apis mellifera L. en colonias del secano costero e interior de la VI Región, Chile. Parasitol. Latinoam. 59:137-141.

INEGI. 2017. Instituto Nacional de Estadística y Geografía. Anuario estadístico y geográfico de Tabasco. 23 p. http://internet.contenidos.inegi.org.mx/contenidos/Productos/prod-serv/ contenidos/espanol/bvinegi/productos/nueva-estruc/anuarios-2017/702825095123.pdf.

Invernizzi, C.; Abud, C.; Tomasco, I. H.; Harriet, J.; Ramallo, G.; Campa, J.; Katz, H.; Gardiol, G. and Mendoza, Y. 2009. Presence of Nosema ceranae in honeybees (Apis mellifera) in Uruguay. J. Invertebrate Pathol. 101(2):150-153.

Jaycox, E. R. 1960. Surveys for Nosema disease in honeybees in California. J. Econ. Entomol. 53:95-98.

Klee, J.; Besana, A. M.; Genersch, E.; Gisder, S.; Nanetti, A.; Tam, D. Q.; Chinh, T. X.; Puerta, F.; Ruz, J. M.; Kryger, P.; Message, D.; Hatjina, F.; Korpela, S.; Fries, I. and Paxton, R. J. 2007. Widespread dispersal of the microsporidian Nosema ceranae, an emergent pathogen of the western honeybee, Apis mellifera. J. Invertebrate Pathol. 96:1-10.

Maggi, M.; Antúnez, K.; Invernizzi, C.; Aldea, P.; Vargas, M.; Negri, P.; Brasesco, C.; De Jong, D.; Message, D.; Weinstein, T. E.; Principal, J.; Barrios, C.; Ruffinengo, S.; Rodríguez, R. D. R. and Eguaras, M. 2016. Honeybee Health in South America. Apidologie. 47:835-854.

Masaquiza, M. D. A.; Curbelo, L. M.; Díaz, M. B. L. y Arenal, C. A. 2019. Varroasis y mecanismos de defensa de la abeja melífera (Apis mellifera). Rev. de Producción Animal. 31(3)2-13.

Martínez, C. M.; Rosas-Córdoba, J.; Prieto, M. D.; Carmona, G. A.; Peña, P. B. y Ávila, R. F. 2016. Presencia de Varroa destructor, Nosema apis y Acarapis woodi en abejas (Apis mellifera) de la región oriente del Estado de México. Abanico Veterinario. 6(2):30-38.

Martínez, P. J. F. y Catzín, V. G. A. 2012. Diagnóstico sanitario de los principales parásitos (Varroa destructor, Nosema apis y Acarapis woodi) en abejas melíferas (Apis mellifera) en la zona centro del estado de Campeche, México. In: XXVI Seminario Americano de Apicultura. Tepic, Nayarit. Organización Nacional de Apicultores. 202-206 pp.

Martínez, J.; Leal, G. and Conget, P. 2012. Nosema ceranae an emergent pathogen of Apis mellifera in Chile. Parasitol. Res. 111:60-607.

Martínez, P. J. F. y Cetzal, I. W. 2018. La importancia de los estudios epidemiológicos en las colonias de abejas melíferas (Apis mellifera) en la península de Yucatán, México. J. Selva Andina Animal Sci. 5(1):1-2.

Martínez, P. J. F.; Medina, M. L. A. y Catzín, V. G. 2011. Frecuencia de Varroa destructor, Nosema apis y Acarapis woodi en colonias manejadas y enjambres silvestres de abejas (Apis mellifera) en Mérida, Yucatán, México. Rev. Mex. Cienc. Pec. 2(1):25-38.

Martínez, P. J. F.; Medina, M. L. A.; Leal, H. M. y Merlo, M. F. E. 2015. Frecuencia de Varroa destructor, Nosema spp. y Acarapis woodi en colonias comerciales de abejas (Apis mellifera) en Yucatán, México. J. Selva Andina Animal Sci. 2(1):2-12.

Medici, S. K.; Sarlo, E. G.; Porrini, M. P.; Braunstein, M. and Eguaras, M. J. 2011. Genetic variation and widespread dispersal of Nosema ceranae in Apis mellifera apiaries from Argentina. Parasitol. Res. 110:859-864.

Medina, F. C. A.; Guzmán, N. E.; Espinosa, M. K. G.; Uribe, R. J. L.; Gutiérrez, L. R. y Gutiérrez, P. F. J. 2014. Frecuencia de varroosis y nosemosis en colonias de abejas melíferas (Apis mellifera) en el estado de Zacatecas, México. Rev. Chapingo Ser. Cienc. Forest. Amb. 20(3):159-167.

Nava, B. M. 1996. Nosemosis en abejas (Apis mellifera) en la región sur del estado de Jalisco, México. Tesis licenciatura. Universidad de Guadalajara. Jalisco, México.

OIE. 2008. Nosemosis de las abejas melíferas. Manual terrestre. Organización Mundial de Sanidad Animal. 388-393 pp.

Paxton, R. J. 2010. Does infection by Nosema ceranae cause ‘colony collapse disorder’ in honeybees (Apis mellifera)? J. Apicultural Res. 49:80-84.

Roy, D.; Debnath, P.; Mondal, D. and Kanti, S. P. 2018. Colony collapse disorder of honeybee: a neoteric ruction in global apiculture. Current J. Appl. Sci. Technol. 26(3):1-12.

Shakib, V. and Mehdi, E. S. 2016. Unprecedented first record of infestation level Acarapis woodi (Rennie) and overwintering ability in Savojbolagh regions of Alborz province in Iran. J. Entomol. Zoology Stud. 4:1-13.

SIAP. 2021. Servicio de Información Agroalimentaria y Pesquera. Base de datos de la actividad agrícola, pecuaria y pesquera en México. http://infosiap.siap.gob.mx/repoAvance-siap-gb/pecAvanceEdo.jsp.

Singh, S.; Makkar, G. S. and Chhuneja, P. K. 2013. Record of parasitic mites associated with different species of honeybees in India. Insect Enviroment. 19:37-42.

Steel, R. G. D. y Torrie, J. H. 1988. Bioestadística: principios y procedimientos. 1ª (Ed.). Español. McGraw Hill, México. 622 p.

Tapia, G. J. M.; Alcazar, O. G.; Macías, M. J. O.; Contreras, E. F.; Tapia, R. J. C.; Chavoya, M. F. J. y González, J. C. 2017. Nosemosis en abejas melíferas y su relación con factores ambientales en Jalisco, México. Rev. Mex. Cienc. Pec. 8(3):325-330.

Thrusfield, M. 2007. Veterinary epidemiology. 3rd (Ed.). Reissued in pbk. with updates. Oxford, Blackwell Science. 610 p.

Van Engelsdorp, D.; Hayes, J.; Hunderwood, R. M. and Pettis, J. 2008. A survey of honeybee colony losses in the USA. Fall 2007 to spring 2008. Public Library of Science. 4071:1-6.

Vaziritaba, S.; Aghamirkarim, A. and Mehdi, S. 2016. Evaluation of the defensive behavior in two honeybee races Iranian honeybee (Apis mellifera meda) and Carniolan honeybee (Apis mellifera carnica) and grooming behavior of different bee races in controlling Varroa destructor mite in honey. J. Entomol. Zoology Stud. 4(5):586-602.

Wilson, W. and Nunamaker, R. 1983. The incidence of Nosema apis Z. in honeybees in Mexico. Bee World. 64(3):132-136.

Downloads

Published

2022-03-23

How to Cite

Martínez Puc, Jesús Froylán, Juan Florencio Gómez Leyva, Nicolás González Cortés, Francisco Javier Catzím Rojas, Yessenia Sánchez Melo, and Emeterio Payró de la Cruz. 2022. “Presence of Varroa Destructor, Nosema Spp. And Acarapis Woodi in Colonies of Bees from Tabasco, Mexico”. Revista Mexicana De Ciencias Agrícolas 13 (2). México, ME:303-15. https://doi.org/10.29312/remexca.v13i2.3033.

Issue

Vol. 13 No. 2 (2022)

Section

Articles

License

Copyright (c) 2022 Revista Mexicana de Ciencias Agrícolas

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

The authors who publish in Revista Mexicana de Ciencias Agrícolas accept the following conditions:

In accordance with copyright laws, Revista Mexicana de Ciencias Agrícolas recognizes and respects the authors’ moral right and ownership of property rights which will be transferred to the journal for dissemination in open access. Invariably, all the authors have to sign a letter of transfer of property rights and of originality of the article to Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) [National Institute of Forestry, Agricultural and Livestock Research]. The author(s) must pay a fee for the reception of articles before proceeding to editorial review.      

All the texts published by Revista Mexicana de Ciencias Agrícolas —with no exception— are distributed under a Creative Commons License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned.

The author(s) can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Revista Mexicana de Ciencias Agrícolas (for example include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Revista Mexicana de Ciencias Agrícolas.

For all the above, the authors shall send the Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a PDF file to: revista_atm@yahoo.com.mx; cienciasagricola@inifap.gob.mx; remexca2017@gmail.

 

This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 International license.

Most read articles by the same author(s)