Abundance and genetic diversity of Fusarium oxysporum and Trichoderma sp. in muse AAB
DOI:
https://doi.org/10.29312/remexca.v10i8.1831Keywords:
banana, populations, CFUAbstract
The occurrence and diversity of Fusarium and Trichoderma species was studied in 29 points of a banana plot (Musa AAB) in Veracruz, Mexico, during November 2017 and April 2018. The plot was divided into two parts; in the first one, fallow and weed removal were carried out (Procedure in culture. 1) while in the second one these activities were not carried out (Procedure in culture; and 2). Fungi were isolated from the soil collected at these sites using potato dextrose and agar (PDA) and K2. At the time of collecting the samples, temperature, precipitation and solar radiation were recorded. The results of the analysis showed 68% more presence of colony forming units (CFU) in soils where no cultivation work was carried out using K2 unlike soils where they were performed using PDA (32%). A small amount of CFU was also observed when the temperature, precipitation and radiation values were high. Molecular analyzes showed 65% more abundance of species in soils without labor compared to cultivated soils (35%), six of 16 Fusarium isolates were Fusarium oxysporum f. sp. melonis and six of 13 Trichoderma isolates were Trichoderma longibrachiatum in the first sampling while nine of 14 Trichoderma isolates were Trichoderma spirale in the second sampling. The analysis of the intrapopulation diversity of seven Trichoderma spirale isolates showed bands of 75 to 500 bp in three loci of the genome in the VB28IT1 isolate with five ISSR.
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