Salicylic acid induces tolerance to cryogenic stress in Solanum tuberosum
DOI:
https://doi.org/10.29312/remexca.v10i7.1627Keywords:
in vitro regeneration, potato, ultra low temperatureAbstract
A common problem in cryogenic processes is survival at ultra low temperature (-196 °C). Based on the physiological effects of salicylic acid (AS) on the signaling of stress tolerance responses, the objective was to evaluate the effect of AS on increasing survival and on the growth of potato buds undergoing cryogenics. Microplants of the advanced clone 06-27 of the Germplasm Bank in vitro of the Potato Program in Metepec State of Mexico, Mexico, were incubated for 28 days in AS (0, 10-6 and 10-5 M) and subsequently subjected to cryogenic process. Effect of AS in plant regeneration: a significant increase in survival was observed in plants pretreated with AS 10-5 and 10-6 M with respect to the control (1.66-2.04 times respectively). Fresh weight, stem length and root significantly increased in microplants pretreated with AS with respect to the control. Cryogenesis: both concentrations of 10-5 and 10-6 M AS induced significantly greater survival (2.17-3.21 times respectively) of explants to cryogenics. Plants preincubated in AS and regenerated after being subjected to cryogenics for 1 h significantly increased root length. The control showed callus formation, absent in the plants treated with AS. The AS-cryogenic combination favored the development of plants subjected to methods that employ ultra-low temperatures in potatoes for the purpose of germplasm cryopreservation or cryotherapy to obtain virus-free materials.
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