Estudio preliminar para la propagación in vitro de Cedrus atlantica mediante yemas axilares
DOI:
https://doi.org/10.29312/remexca.v7i8.140Keywords:
cedro, brotación, micropropagación, plantas leñosasAbstract
Cedrus atlantica es una planta ornamental utilizada para la decoración de parques y jardines. Con el fin de micropropagar material de calidad de esta planta, se implementó un protocolo de multiplicación in vitro. Para ello, como explantes se usaron las yemas axilares que se desinfestaron con hipoclorito de sodio al 10% por 15 min, tratamiento con lo que se obtuvo un 83% de material aséptico. El cultivo de los explantes se realizó en el medio basal McCown’s Woody Plant (WPM) adicionado con carbón activado (1 g L-1) y cisteína (25 mg L-1), lo que permitió obtener cultivos sin rasgos de oxidación. En la etapa de multiplicación con el uso de la bencil amino purina (5 mg L-1) se obtuvieron hasta 5 brotes por explante, que presentaron un crecimiento de 1.4 cm. Sin embargo, el ácido indol butírico a 2.5 y 5 mg L-1 no indujo la formación de raíces en los brotes in vitro.
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