Outbreak induction from floral stem orchid Phalaenopsis spp. (Blume) in vitro

Authors

  • Karla Andrea Frausto Jaime Facultad de Agronomía-Universidad Autónoma de Nuevo León-Unidad Marín. Carretera Zuazua-Marín km 17.5, Marín, Nuevo León, México. CP. 66700. Tel. 81 13404399
  • Ma. del Carmen Ojeda Zacarías Facultad de Agronomía-Universidad Autónoma de Nuevo León-Unidad Marín. Carretera Zuazua-Marín km 17.5, Marín, Nuevo León, México. CP. 66700. Tel. 81 13404399
  • Omar Guadalupe Alvarado Gómez Facultad de Agronomía-Universidad Autónoma de Nuevo León-Campus Ciencias Agropecuarias. Francisco Villa s/n, Col. Ex-Hacienda El Canadá, Gral. Escobedo, Nuevo León, México. CP. 66050. Tel. 81 13404399
  • Eduardo Alejandro García Zambrano Facultad de Agronomía-Universidad Autónoma de Nuevo León-Campus Ciencias Agropecuarias. Francisco Villa s/n, Col. Ex-Hacienda El Canadá, Gral. Escobedo, Nuevo León, México. CP. 66050. Tel. 81 13404399
  • Humberto Rodríguez Fuentes Facultad de Agronomía-Universidad Autónoma de Nuevo León-Campus Ciencias Agropecuarias. Francisco Villa s/n, Col. Ex-Hacienda El Canadá, Gral. Escobedo, Nuevo León, México. CP. 66050. Tel. 81 13404399
  • Gilberto Rodríguez Pérez Instituto Tecnológico de Roque. Carretera Celaya-Juventino Rosas km 8. Celaya, Guanajuato. CP. 38110. Tel. 461 6115903

DOI:

https://doi.org/10.29312/remexca.v10i6.608

Keywords:

Phalaenopsis, micropropagation, organogenesis

Abstract

The induction of outbreaks in vitro is a strategy of the vegetable micropropagation used to increase the production of orchids, in comparison with the traditional crop that requires a long period of time to obtain a substantial population for commercialization. The objective of this study was to induce in vitro outbreak formation from the flower outbreaks of the orchid Phalaenopsis spp., using an organogenesis route. Four mineral salt formulations (MS 100%, MS 50%, Knudson and NPK Fertilizer 18-9-18/15-30-15) and four varieties of Phalaenopsis spp. to determine the viability of explants. Two growth regulators (6.2-benzylaminopurine BAP 20.2 µM alone and combined with naphthalenacetic acid ANA 5.37 µM) were evaluated to determine outbreak induction. The results indicate that oxidation in explants depends on the mineral salts of the culture media, as well as the variety of orchid; outbreak induction depends on the growth regulator used. BAP/ANA regulators produced 2.4 outbreaks per outbreak against 2 in BAP. The MS 50% medium proved to be the best option to maintain the viability of the flower outbreaks.

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Published

2019-09-23

How to Cite

Frausto Jaime, Karla Andrea, Ma. del Carmen Ojeda Zacarías, Omar Guadalupe Alvarado Gómez, Eduardo Alejandro García Zambrano, Humberto Rodríguez Fuentes, and Gilberto Rodríguez Pérez. 2019. “Outbreak Induction from Floral Stem Orchid Phalaenopsis Spp. (Blume) in Vitro”. Revista Mexicana De Ciencias Agrícolas 10 (6). México, ME:1207-18. https://doi.org/10.29312/remexca.v10i6.608.

Issue

Vol. 10 No. 6 (2019)

Section

Articles

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