Study of genetic diversity in Indigofera suffruticosa crops in El Salvador
DOI:
https://doi.org/10.29312/remexca.v15i8.3557Keywords:
Indigofera suffruticosa, haplotypic diversity, ITS2 ribonuclear regionAbstract
Añil is a woody shrub that belongs to the Fabaceae family. It is recognized as native and contributes to the sustainable agriculture of many rural regions of El Salvador due to its ability to fix nitrogen to the soil and is preferred because it produces quality indigotine, which is exported to different parts of the world. Currently, its genetic status is unknown; this research aimed to analyze the genetic variability of añil in crops in El Salvador. The samples were collected in five locations with cultivars of the species in 2021. DNA extraction was performed using the CETAB protocol with modifications, followed by PCR amplification of the ITS2 ribonuclear region. The PCR products were sent to Macrogen (Korea) for sequencing. The sequences were edited and analyzed using bioinformatics programs. The analyses revealed two haplotypes distributed in the Salvadorean territory and low genetic diversity indices (Hd= 0.46, π= 0.004). In addition, very high genetic differentiation has been found, mainly between the CPB locality and the rest of the localities (Fst≥ 0.5). The results are conclusive regarding a low genetic diversity, the presence of very few haplotypes, and a gene structure based on differences mostly between populations.
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