Basic seed production of cassava clone Agrosavia Melúa-31
DOI:
https://doi.org/10.29312/remexca.v16i1.3498Keywords:
tissue culture, micropropagation, plant health, asexual seedAbstract
The cassava frogskin disease (CFSD) is one of the main phytosanitary constraints for the sustainable production of cassava because it restricts the tuberization and obtaining of healthy planting material from commercial plantations. The cassava clone Agrosavia Melúa-31 is of high socioeconomic relevance for the Colombian Orinoquía region; nevertheless, the high susceptibility to cassava frogskin disease restricts the commercial planting of this clone. Therefore, this research was conducted with the aim of developing a preliminary protocol for the production of basic seed of the cassava clone Agrosavia Melúa-31. This work was carried out at Agrosavia, Turipaná Research Center in three phases: in vitro micropropagation, ex vitro acclimatization and hardening, and adaptation to field conditions. The results revealed that, under in vitro growing conditions, the survival rate of the Agrosavia Melúa-31 clone ranged from 85 to 90%. In the acclimatization phase, the highest percentage of survival (87.5%), fresh (2.6 g) and dry (0.42 g) biomass production, and leaf area index (0.66) were obtained with a substrate based on sand, organic matter, and alluvium. In the phase of adaptation to field conditions, the survival rate was 98%, healthy roots and basic seed (Cangres) of Agrosavia Melúa-31 of high phytosanitary and genetic quality were harvested. The standardization of quality seed production protocols is an indispensable tool for the sanitization of vegetatively propagated materials.
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