Distillates and homeopathic oil of Tagetes remotiflora: effect on Botrytis cinerea
DOI:
https://doi.org/10.29312/remexca.v16i2.3494Keywords:
Tagetes remotiflora, fungal disease, hydrolate, inhibitionAbstract
A phytogenetic resource in Mexico is Tagetes remotiflora Kunze, with potential for the control of fungal diseases. The objective was to describe the composition of T. remotiflora essential oil and to evaluate distillates (oil and hydrolate) and homeopathic essential oil on the control of Botrytis cinerea in vitro in 2021. Essential oil and hydrolate were obtained by hydrodistillation of flowering plants, the essential oil was also prepared in its homeopathic form, and the oil was analyzed using the GC-MS technique. The treatments evaluated were PDA medium (39 g L-1), Cabrio C fungicide (1 g L-1), Tween 20 (0.1 ml L-1), essential oil (0.0001, 0.001, 0.01, 0.1, 0.5, 1 and 2%), hydrolate (25, 50, 75, and 100%), and homeopathic essential oil (1, 2, 3, 4, 5, 6, 60 and 200 CH). The data on the percentage of inhibition and mycelial growth rate were subjected to an analysis of variance and Tukey’s test (p≤ 0.05) and minimum inhibitory concentration 50 (MIC50) and 90% (MIC90) were determined by probit analysis. Thirty-seven chemical compounds were identified, four of which were the majority: trans-β-Ocimene, 2-Carene, Cyclohexene, 1-methyl-4-(1-methylethylidene)- and 5,7-Octadien-4-one, 2,6-dimethyl-, (E)-. Mycelial growth was inhibited by 92% with the 0.5% essential oil, and by 100% with 1 and 2%, as with the fungicide; with the homeopathic oil 6 CH, the inhibition was 19.2% and with 75 or 100% hydrolate, the inhibition was from 74 to 85%. The probit analysis confirms the biocidal effect of the essential oil and the fungistatic effect of the hydrolate against B. cinerea.
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