Identification of five strains of Simplicillium associated with pustules of coffee rust in Costa Rica

Authors

  • Gerardo García-Nevarez Campo Experimental Delicias-INIFAP. Carretera Delicias-Rosales km 2, Delicias, Chihuahua. CP. 33000
  • Leslie Carnero-Avilés Campo Experimental Delicias-INIFAP. Carretera Delicias-Rosales km 2, Delicias, Chihuahua. CP. 33000
  • Eduardo Hidalgo-Jaminson Centro Internacional de Agricultura y Biociencias. Turrialba, Cartago, Costa Rica. CP. 30501

DOI:

https://doi.org/10.29312/remexca.v16i1.3392

Keywords:

cultural characterization, natural biological control, physiological characterization

Abstract

Coffee rust (Hemileia vastatrix) is one of the most economically important diseases in coffee crops due to the production losses of up to 50% that it causes. In addition to the conventional chemical control of this disease, several natural enemies, such as mycoparasitic fungi, regulate its incidence and severity. The study aimed to identify and characterize five strains of fungi associated with the natural control of H. vastatrix isolated from the coffee-growing area of Turrialba, Cartago, Costa Rica. Five strains of Simplicillium were identified by molecular analysis; measurements of macroscopic characteristics, microscopic characteristics, radial growth, and conidia production were made. According to the analysis in the NCBI database, the five strains matched the species Simplicillium lanosoniveum. Overall, macroscopic characteristics were similar between the isolates; the most notable difference was the coloration of the culture medium. Strains that showed a color change from white to brown had lower radial growth. The arrangement of the phialides, the shape and size of the conidia, and the microscopic features were generally consistent with the genus Simplicillium. Conidia size and production were statistically different between strains (p˂ 0.0001). In addition, the strains with the largest conidia recorded the lowest values of conidia production. These results provide the first report that characterizes strains of the genus Simplicillium associated with H. vastatrix in Costa Rica.

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Published

2025-03-20

How to Cite

García-Nevarez, Gerardo, Leslie Carnero-Avilés, and Eduardo Hidalgo-Jaminson. 2025. “Identification of Five Strains of Simplicillium Associated With Pustules of Coffee Rust in Costa Rica”. Revista Mexicana De Ciencias Agrícolas 16 (1). México, ME:e3392. https://doi.org/10.29312/remexca.v16i1.3392.

Issue

Vol. 16 No. 1 (2025)

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Articles

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