Regeneration of leaf explants of five raspberry genotypes
DOI:
https://doi.org/10.29312/remexca.v14i6.3183Keywords:
Rubus idaeus L., organogenesis, plant hormonesAbstract
The efficiency in raspberry regeneration, from leaf explants, is limited due to several factors, among which the age of the explant and the genotype stand out. The aim of this research was to determine the effect of growth regulators on oxidation and in vitro regeneration from leaf explants of five raspberry genotypes in 2021. Doses and combinations of auxins and cytokinins were tested to induce direct organogenesis in leaf explants of raspberry genotypes; ‘C-6’, ‘Joan J.’, ‘A-1’, ‘UM-702’ and ‘Heritage’. The results showed that the regulator benzylaminopurine (BAP) decreased oxidation in genotypes ‘C-6’, ‘Joan J.’, ‘A-1’ and ‘Heritage’ by 36, 48, 60 and 68%, respectively, those that were supplemented with kinetin had a reduction in oxidation in the genotype ‘C-6’ (56%), when thidiazuron (TDZ) was added, oxidation decreased in the genotypes evaluated by 72, 64, 72, 84 and 68%, respectively. The greatest regeneration (number of shoots/explant) was with BAP (0.5 mg L-1) and TDZ (0.2 mg L-1) + indole butyric acid (IBA) (0.1 mg L-1) for the genotype ‘C-6’, and TDZ (0.2 mg L-1) + IBA (0.1 mg L-1) for ‘Joan J.’ and ‘Heritage’. In ‘A-1’ and ‘UMC-702’, the use of TDZ (0.2 mg L-1) alone is suggested. It is concluded that the use of growth regulators, alone or combined, decreases oxidation in leaf explants, and increases the survival and regeneration of shoots in all genotypes evaluated.
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