First report of pokkah boeng in sugarcane in the Huasteca Potosina

Authors

  • Fabiola Medina-Osti Faculty of Agronomy-Autonomous University of Nuevo León. Francisco Villa s/n, Colonia Ex-Hacienda El Canada, General Escobedo, Nuevo León. CP. 66054. Tel. 8113404399, ext. 3517
  • Adriana Gutiérrez-Díez Faculty of Agronomy-Autonomous University of Nuevo León. Francisco Villa s/n, Colonia Ex-Hacienda El Canada, General Escobedo, Nuevo León. CP. 66054. Tel. 8113404399, ext. 3517
  • Salvador Ochoa-Ascencio Faculty of Agrobiology ‘Presidente Juárez’-Michoacan University of San Nicolás of Hidalgo. Paseo General Lázaro Cárdenas and Berlin s/n, Viveros neighborhood, Uruapan, Michoacán. CP. 60170. Tel. 452 5236474
  • Enrique Ignacio Sánchez-González Federal University of Lavras-Department of Phytopathology. Lavras, MG, Brazil. CP. 37200-900. Tel. 55 3538291122

DOI:

https://doi.org/10.29312/remexca.v13i7.2998

Keywords:

Saccharum officinarum, Fusarium sacchari, twisted top

Abstract

Sugarcane (Saccharum officinarum) is one of the main crops produced in the world, Mexico is the sixth producer worldwide, while San Luis Potosí ranks third in production nationwide. Sugarcane plants with wilt symptoms similar to those caused by pokkah boeng disease were collected in sugarcane fields of the Huasteca Potosina. This disease known as twisted top is caused by some species of the genus Fusarium and causes economic losses due to the decrease in the quality of the harvested crop. Two isolates of fungi with morphological characteristics typical of Fusarium sp. were obtained from the stems of these plants. The identification of the isolates was carried out through the morphological characteristics of the macroconidia, microconidia and the characteristics of the colony. The molecular identification of the species was carried out by sequencing the regions of the genes ITS, β-tubulin (BT) and elongation factor (TEF). Koch’s postulates were fulfilled for the isolates obtained by inoculation in the sugarcane varieties My 55 and Mex 79-431. The morpho-molecular characterization of the isolates identified Fusarium sacchari as the causative agent of the disease. As far as is known, this is the first report of Fusarium sacchari as a causative agent of pokkah boeng disease in sugarcane in the Huasteca Potosina region.

Downloads

Download data is not yet available.

References

Arcudia, C. E.; Flores, H.; Orta, S. B. y Torres, B. 2018. Agricultura industrial en la Huasteca Potosina: la caña de azúcar. México. Tlatemoani: Rev. Académica de la Investigación. 9(27)131-146.

Carbone, I. and Kohn, L M. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia. United States. 91(3)553-556.

Cenis, J. L. 1992. Rapid extraction of fungal DNA for PCR amplification. Nucleic acids research. United Kingdow. 20(9):2380. Doi:10.1093/nar/20.9.2380.

Chala, A.; Degefu, T. and Bente, B. M. 2019. Phylogenetically diverse Fusarium species associated with sorghum (Sorghum bicolor L. Moench) and finger Millet (Eleusine coracana L. Garten) grains from Ethiopia. Diversity. Switzerland. 11(93):1-11. Doi:10.3390/d11060093.

Dohare, S.; Mishra, M. M. and Kumar, B. 2003. Effect of wild on juice quality of sugarcane. Annals of Biology. India. 19(2):183-186.

FAO. 2022. Food and Agriculture Organization of the United States. FAOSTAT. División de Estadística. https://www.fao.org/faostat/en/#data/QCL.

Govender, P.; McFarlane, S. A. and Rutherford, S. R. 2010. Fusarium species causing pokkah boeng and their effect on Eldana saccharina walker (Lepidoptera: Pyralidae). In: Proceedings South Africa Sugar Technologists Association. 83rd. SASTA Congress. South African Sugar Technologists Association. Durban, South Africa. 267-270 pp. Jeyakumar, J. M. J. and Zhang, M. 2020. Symptoms and their assessment of sugarcane pokkah boeng. Int. J. Environ. Agric. Res. United States of America. 6(12):50-54. https://ijoear. com/assets/articles-menuscripts/file/IJOEAR-DEC-2020-15.pdf.

Leslie, J. F. and Summerell, B. A. 2006. The Fusarium laboratory manual. Blackwell Publishing. First (Ed.). Ames, Iowa, United States of America. 240-241 pp.

Lin, Z. X. S.; Que, Y.; Wang, J.; Comstock, J. C.; Wei, J.; McCord, P. H.; Chen, B.; Chen, R. and Zhang, M. 2014. Species-specific detection and identification of Fusarium species complex, the causal agent of sugarcane pokkah boeng in China. PLoS One. 9(8):e104195. doi:10.1371/journal.pone.0104195.

López, E. 2005. Variedades promisorias de caña de azúcar (Saccharum spp.) para la Huasteca Potosina. Fundación Produce de San Luis Potosí, AC. San Luis Potosí, SLP. México. Folleto núm. 1. 34 p.

Marques, J. P. R.; Soares, M. K. M. and Appezzato, G. B. 2013. New staining technique for fungal-infected plant tissues. Turkish J. Bot. 37(4):1-4. Doi:10.3906/bot-1204-9.

Martínez, F. E.; Martínez, J. P.; Guillén, D.; Peña, Ch. G. y Hernández, H. V. M. 2015. Diversidad de Fusarium en las raíces de caña de azúcar (Saccharum officinarum) en el estado de Morelos, México. Rev. Mex. Micol. 42:33-43. http://www.scielo.org.mx/scielo.php? script=sci-arttext&pid=S0187-31802015000200006.

Nordahliawate, M. S.; Nur Ain Izzati, M. Z.; Azmi, A. R. and Salleh, B. 2008. Distribution, morphological characterization and pathogenicity of Fusarium sacchari associated with pokkah boeng disease of Sugarcane in Peninsular Malaysia. Pertanika J. Trop. Agric. Sci. Malaysia. 31(2):279-286. https://core.ac.uk/download/pdf/153798779.pdf.

O’Reilly, G. 1998. The South African sugar industry. United Kingdom. Int. Sugar J. 100:266-268.

Patil, A. S.; Singh, H.; Sharma, S. R. and Rao, G. 2007. Morphology and pathogenicity of isolates of Fusarium moniliforme causing pokkah boeng disease of sugarcane in Maharashtra. In: microbial diversity: modern trends. Ram, R. C. and Sinha, A. (Ed.). Daya Delhi Ed. New Delhi, India. 234-263 pp.

Rosas, G. V.; Hernández, A. M.; Miranda, M. R.; Bravo, M. E. y Berriozabal, O. A. 2014. Identificación y variabilidad morfológica de pokkah boeng (Fusarium spp.) en caña de azúcar. Investigación Agropecuaria. México. 11(2):119-126. https://investigacionagropecu aria.jimdofree.com/art%C3%ADculos-11-2/.

SIAP. Sistema de Información Agroalimentaria y Pesquera. 2021. Anuario estadístico de producción agrícola. Cierre de la producción agrícola. https://nube.siap.gob.mx/cierre agricola/.

Singh, A.; Chauhan, S. S.; Singh, A. and Singh, S. B. 2006. Deterioration in sugarcane due to pokkah boeng disease. Sugar Tech. India. 8(2-3):187-190. https://link.springer.com/ article/10.1007/BF02943659.

Stothard, P. 2000. The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences. BioTechniques 28(6):1102-1104. Doi: 10.2144/00286ir01.

Viswanathan, R.; Balaji, C. G.; Selvakumar, R.; Malathi, P.; Ramesh, S. S. A.; Naveen, P. C.; Chhabra, M. L. and Parameswari, B. 2017. Epidemiology of Fusarium diseases in sugarcane: a new discovery of same Fusarium sacchari causing two distinct diseases, wilt and pokkah boeng. Sugar Tech. India. 19(6):638-646. Doi: 10.1007/s12355-017-0552-4.

White, T. J.; Bruns, T.; Lee, S. and Taylor, J.1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. Innis, M. A; Gelfand, D. H; Sninsky, J. J. and White, T. J. (Ed.). Academic Press. California, USA. 315-322 pp. https://doi.org/10.1016/B978-0-12-372180-8.50042-1.

Wishwakarma, S. K.; Kumar, P.; Nigam, A.; Singh, A. and Kumar, A. 2013. Pokkah boeng: an emerging disease of sugarcane. J. Plant Pathol. Microbiol. Brussels, Belgium. 4(3):1000170. Doi: 10.4172/2157-7471.1000170. Zhang, M. and Jeyakumar, J. M. J. 2018. Fusarium species complex causing pokkah boeng in China. In: Fusarium: plant diseases, pathogen diversity, genetic diversity, resistance and molecular markers. Askun, T. (Ed.). IntechOpen. United Kingdom. 139-154 pp. Doi: 10.5772/intechopen.73133.

Published

2022-11-22

How to Cite

Medina-Osti, Fabiola, Adriana Gutiérrez-Díez, Salvador Ochoa-Ascencio, and Enrique Ignacio Sánchez-González. 2022. “First Report of Pokkah Boeng in Sugarcane in the Huasteca Potosina”. Revista Mexicana De Ciencias Agrícolas 13 (7). México, ME:1307-13. https://doi.org/10.29312/remexca.v13i7.2998.

Issue

Section

Investigation notes

Most read articles by the same author(s)