In vitro morphogenesis of Mammillaria plumosa Weber

Authors

  • Janeth Téllez-Román Posgrado en Recursos Genéticos y Productividad-Fisiología Vegetal-Colegio de Postgraduados. Carretera México-Texcoco, km 36.5. Montecillo, Texcoco, Estado de México, México. CP. 56230
  • María Cristina Guadalupe López-Peralta Posgrado en Recursos Genéticos y Productividad-Genética-Colegio de Postgraduados. Carretera México-Texcoco, km 36.5. Montecillo, Texcoco, Estado de México, México. CP. 56230
  • Eleodoro Hernández-Meneses Posgrado en Recursos Genéticos y Productividad-Fisiología Vegetal-Colegio de Postgraduados. Carretera México-Texcoco, km 36.5. Montecillo, Texcoco, Estado de México, México. CP. 56230
  • Andrés Adolfo Estrada Luna CINVESTAV-Unidad Irapuato-Departamento de Ingeniería Genética
  • Hilda Araceli Zavaleta Mancera osgrado en Botánica- Colegio de Postgraduados. Carretera México-Texcoco, km 36.5. Montecillo, Texcoco, Estado de México. CP. 56230. Tel. 01(595) 9520200, ext. 1540
  • Manuel Livera Muñoz Posgrado en Recursos Genéticos y Productividad-Genética-Colegio de Postgraduados. Carretera México-Texcoco, km 36.5. Montecillo, Texcoco, Estado de México, México. CP. 56230

DOI:

https://doi.org/10.29312/remexca.v8i4.13

Keywords:

barrel cactus, cactus, micropropagation, organgenesis, 2, 4-D

Abstract

Mammillaria plumosa is a highly appreciated Mexican  cactus as an ornamental plant for its peculiar morphology.  Excessive extraction and looting of its wild populations  have forced its protection and is currently classified as  an endangered species. Given this critical situation this  paper aimed at developing an efficient in vitro propagation  system feasible to be implemented as a strategie for  recovering the species. Stem segments with areolae were  seeded in medium MS (1962), supplemented with 2,4-D  (9, 13.5 and 18 μM) in combination with kinetin (4.6, 9.3  and 13.9 μM). All evaluated treatments induced callus  formation but the activation of the areolae for conversion  to shoots was only obtained with 18 μM of 2,4-D and 9.3  μM of kinetin. In the proliferation stage callus continued  to grow at the same concentrations of growth regulators,  shoot differentiation occurred from the areolar activation  and differentiation of novo adventitious buds. Shoots  formed roots naturally in the process but the rooting was  improved with the culture in medium MS (1962) at half  the concentration of salts. In the plants acclimatization  the survival rate was 85% in substrate of peat and river sand. Using this protocol it is possible to establish the  micropropagation of Mammillaria plumosa where 500  plants can be regenerated in an average of 24 weeks of  culture, which could be used in restoring wild populations  or for commercial use. 

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Vol. 8, Núm 4

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Published

2017-08-04

How to Cite

Téllez-Román Janeth, López-Peralta María Cristina Guadalupe, Hernández-Meneses Eleodoro, Estrada Luna Andrés Adolfo, Hilda Araceli Zavaleta Mancera, and Livera Muñoz Manuel. 2017. “In Vitro Morphogenesis of Mammillaria Plumosa Weber”. Revista Mexicana De Ciencias Agrícolas 8 (4). México, ME:863-76. https://doi.org/10.29312/remexca.v8i4.13.

Issue

Vol. 8 No. 4 (2017)

Section

Articles

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