In vitro evaluation of methods against Botrytis cinerea
DOI:
https://doi.org/10.29312/remexca.v11i3.2077Keywords:
antifungal effect, fungal diseases, homeopathic substances, organic and biological methods, strawberryAbstract
Although experimental methods are available for the control of fungal diseases in strawberry cultivation, including chemical, organic and biological, other low-cost methods such as homeopathic substances are not ruled out, which have not been evaluated against Botrytis cinerea. The objective of the present study was to compare conventional treatments and homeopathic substances, under in vitro conditions, in the mycelial growth of B. cinerea as a previous reference to in vivo evaluation. Treatments: PDA medium, synthetic fungicide Switch® (Fludioxonil, Ciprodinil) 1 g L-1, essential oil of Tagetes lemmonii (1%), commercial extract of Larrea tridentata (2 mL L-1), homeopathic substances, arsenic 6 CH and nosode of Botrytis 7 CH and dual test with Trichoderma harzianum. Registered variables: mycelial diameter, growth rate and percentage of mycelial inhibition; in the dual test, days at first hyphal contact and type of antagonism were measured. The data were subjected to analysis of variance and multiple means comparison test (Tukey, p≤ 0.05). Switch® and Tagetes oil inhibited mycelial growth by 100%, with Larrea extract the inhibition was 65.8%, T. harzianum inhibited 36.2% and hyphal contact occurred on the sixth day (type II antagonism), controlling the pathogen, the homeopathic substances nosode 7 CH and arsenic 6 CH inhibited the fungus in 31.2 and 11.8%, respectively. The effect of nosode 7 CH was different (p≤ 0.05) from the control, but homeopathic arsenic was not.
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