elocation-id: e2936
The consumer is increasingly interested in their state of health and well-being, so the demand for functional foods has increased. The impregnation of bioactive compounds in fruit and vegetable products is a recent technology that opens a door of opportunity to a more demanding market, so a bibliographic review of the latest studies provides an overview for future work on the subject. The impregnation of bioactive compounds in the porous fraction of fruits and vegetables is achieved by osmotic dehydration (OD). In this sense, knowing the OD factors that determine the impregnation of bioactive compounds in plant tissues, their physicochemical stability during storage, and the latest trends in osmo-dehydrated fruit and vegetable products that could be considered functional foods is very important. Therefore, this review considered scientific information from different databases and was organized into three sections that are discussed: fundamentals of the OD, fruit and vegetable products enriched with bioactive compounds, and the physicochemical stability of these products during storage.
dehydrated foods, food stability, functional foods, impregnation.
Fruits and vegetables are an essential part of the human diet as they are a source of bioactive compounds such as vitamins, minerals, phytosterols, and dietary fiber, among others. Generally, fruits and vegetables are marketed fresh, however, their shelf life is limited due to their high metabolic activity, susceptibility to mechanical damage, and attack by microorganisms, which accelerate their senescence and death (Al-Tayyar et al., 2020).
Postharvest losses of fruits and vegetables represent between 40 and 50% of total food losses (Ferreira dos Santos et al., 2020). In this sense, dehydration is one of the most common conservation techniques since it reduces water activity (aw) below 0.7. Dehydration methods include sun drying, hot air drying, vacuum drying, and freeze drying (Qiu et al., 2019).
In recent years, these treatments have been complemented with osmotic dehydration (OD), a pretreatment and process in which plant tissues are immersed in a hypertonic solution at a certain temperature and time, altering in a controlled manner the microstructural characteristics of the plant matrix, in which it is possible to impregnate bioactive compounds (minerals, vitamins, antioxidants, probiotics, fibers, etc.) in its porous fraction, which generates an additional benefit for the consumer.
OD reduces the adverse effects of heat from subsequent processes, improving product color, texture, and flavor, and minimizes production costs by maximizing energy efficiency (Ahmed et al., 2016). The following has been reported: impregnation of calcium and vitamins C and E in potato (Solanum tuberosum var. Diacol Capiro) (Duarte-Correa et al., 2020), the addition of antioxidants from beet juice in apple (Malus domestica L. cv. Granny Smith) (Aguirre-García et al., 2020), probiotics such as Lactobacillus plantarum in apple var. Royal Gala (Emser et al., 2017) and Lactobacillus rhamnosus in banana (Musa paradisiaca var. Tabasco) (Huerta-Vera et al., 2017), among others.
Therefore, this review analyzes the factors of osmotic dehydration that determine the incorporation of bioactive compounds in plant matrices; as well as recent trends in osmo-dehydrated fruit and vegetable products that could venture as functional foods and their physicochemical stability during storage.
OD is a processing technique that involves the immersion of food matrices in a hypertonic solution that induces three flows, i) the transfer of water from the product to the hypertonic solution, ii) the migration of osmotic solute into the product; and iii) leaching of cellular components of the product (sugars, acids, minerals, vitamins) into the hypertonic solution (Figure 1) (Ahmed et al., 2016; González-Pérez et al., 2019).
The factors that determine the overall properties of mass transport during the osmotic process are.
The increase in temperature during OD promotes mass transfer by decreasing the viscosity of the osmotic medium and increasing the permeability of membranes. When the process temperature exceeds the sensitivity of the product, excessive softening, enzymatic darkening, and loss of flavor and aroma occur (Xiao et al., 2018).
It consists of high molecular weight solutes (sucrose) in high concentration, which optimizes the water loss (WL) of the product at the beginning of the process. When low molecular weight (glucose, fructose, sorbitol, etc.) and low concentration solutes are applied, solids gain (SG) is favored over WL (Xiao et al., 2018).
The geometry and size of the product affect the specific surface (surface/volume ratio), a high specific surface favors solids gain (SG) in the product, while to generate greater WL and SG, it is necessary to have a greater surface area and smaller volume of the product (González-Pérez et al., 2019). Likewise, the use of high ratios of hypertonic solution: food (≥ 20:1) prevents the dilution of the osmotic medium and the decrease of the concentration gradient during the process.
On the other hand, the use of stirring during the process ensures continuous contact of the product with the renewed hypertonic solution, which favors SG and WL; however, solids could form a thin layer on the tissue and act as a barrier that hinders subsequent WL of the product (Ahmed et al., 2016).
The species, variety, and state of maturity define the complex and heterogeneous cellular structure of biological materials, with various physicochemical properties (fiber orientation, size of intercellular spaces, cell interconnectivity, pore space, soluble solids, water, etc.) that can facilitate or hinder the flow of mass during the osmotic process.
The pore space, or effective porosity (ɛe), is the fraction of the total volume of the plant matrix occupied by gas. Lech et al. (2018) analyzed the effect of ɛe on mass transfer during the OD of different fruit and vegetable species, showing that products with high ɛe, such as apple (var. Champion), parsley (Petroselinum crispum var. Eagle), and black radish (Raphanus sativus var. Sativus var. Kulata Cerna), present values of 26.75, 22.64 and 20.62%, respectively, which allows them a greater mass transfer during the osmotic process.
Conversely, products such as beet (Beta vulgaris var. Alto) and carrot (Daucus carota var. Nerac.) with a lower ɛe of 4.16 and 3.45%, respectively, presented lower mass transfer during the OD.
In this sense, Sulistyawati et al. (2018) report the effect of the maturity state of the fruit on the ɛe during OD in cubes of mango (Mangifera indica var. Kent.) treated with a solution of sucrose (60 °Brix), pectin methylesterase (PME) and calcium, reporting a higher SG in immature mango because it has more pore space compared to mature mango. Therefore, it is important to consider that the maturation process is not uniform in the product since there are different cell domains or clusters that contribute to the heterogeneity and anisotropy of the tissue, which will affect the OD process.
Fruit and vegetable products have a complex composition distributed in four phases: solid matrix (cell wall, plasmalemma, tonoplast, and cell organelles in the cytoplasm), extracellular liquid phase, intracellular liquid phase and gas. A plant matrix contains up to 80% of spliced and interconnected parenchymal cells, with porous and thin cell wall (CW), a cytoplasm delimited by a membrane and a large central vacuole that can occupy up to 90% of a mature cell where water and nutrients are stored.
The CW is composed of microfibrils of cellulose, hemicellulose, and pectin that form intercellular spaces that confer certain mechanical properties to individual cells, such as stiffness. In particular, pectin has specific functions such as cell-cell adhesion and regulation of CW porosity (Winisdorffer et al., 2015).
Three different routes have been described for mass flow through cell tissues during OD, i) apoplastic transport that takes place within the continuity of cell walls; ii) symplastic transport between adjacent cells via plasmodesmata; and iii) transmembrane transport; where water diffusion is specifically mediated by aquaporins (proteins specialized in water transport) (Mauro et al., 2016).
During the OD of plant matrices, the parenchymal cells of the mesocarp go through three stages derived from the changes in volume (Vt/Vo) and moisture content (Xt/Xo) of the product (Figure 2) (Seguí et al., 2012). In stage 1, when the fresh plant matrix (Figure 2a) is immersed in a hypertonic solution, the chemical potential gradient generates the WL of the protoplast, which contracts and deforms along with the CW until the stretching forces result in incipient plasmolysis, the critical point where the plasma membrane begins to detach from the CW, consequently there is a cell shrinkage (Figure 2b).
During stage 2, mass flow through the plasma membrane increases the driving forces of stretching between the membrane and the CW until the membrane detaches and contracts elastically, generating new intracellular spaces that will be occupied by the hypertonic solution, thus promoting WL, which resulted in a decrease in moisture and volume in the product (Figure 2c).
Finally, in stage 3, the cell structure began to collapse derived from the complete separation between the plasma membrane and the CW, which allowed the protoplast to shrink freely. However, the CW and the Hechtian strands (thin cytoplasmic threads) continue to act as physical barriers to mass flow. At this stage, there was a slight reduction in volume; nevertheless, the entry of hypertonic solution into the cells caused the decrease in moisture content to continue (Figure 2d).
The modifications that occur in the cellular architecture of the plant matrix derived from WL and SG during OD are reflected macroscopically in the physicochemical properties of the product. Figure 3 presents three fruit and vegetable products osmo-dehydrated in sucrose solution (50 °Brix at 35 °C for up to 240 min), where it was appreciated that the elimination of water, in addition to reducing the moisture content and aw of the product, also reduces its size and volume depending on the processing time.
Depending on the composition of the hypertonic solution, the SG will modify the color, flavor, and nutritional and functional properties of the product. On the other hand, the WL concentrates the pigments that provide color to the vegetable matrix, which can be seen as an increase in the purity and intensity of the color of the osmo-dehydrated product with respect to the fresh product.
In the case of melon, there is a transparency derived from the total or partial degassing of the air present in its tissue, which is replaced by the hypertonic solution; on the contrary, in apple and chayote, this transparency is not present, which may be due to the progressive formation of a sucrose bark on their surface.
Together, WL and SG decrease the elasticity and porosity of the product, which determine the texture characteristics of the product, resulting in a firm exterior and soft interior (Barragán-Iglesias et al., 2018).
Functional foods are products modified by removing any of their components or integrating some bioactive compound (dietary fiber, oligosaccharides, polyols, peptides and proteins, isoprenoids and vitamins, lactic acid bacteria, minerals, unsaturated fatty acids, phytochemicals, and antioxidants) that has been scientifically proven to produce a benefit for the consumer (Fuentes-Berrio et al., 2015).
The OD allows altering in a controlled manner the microstructural characteristics of plant matrices by conferring the introduction of preservatives, flavorings, texture-enhancing agents, or bioactive compounds in their porous fraction through the use of multicomponent hypertonic solutions. Derived from this, the OD has been used for the development of new fruit and vegetable products.
Dehydrated slices of landrace mango and impregnated them with emulsions of oleoresin from piquín chili (Capsicum annuum L. var. Aviculare), which showed an antiproliferative effect on breast cancer cell lines MDA-MB-231, attributed to the synergy of capsaicin from piquín chili and bioactive components from mango pulp (Jiménez-Hernández et al., 2017).
Similarly, Shukla et al. (2019) dehydrated slices of mango cv. Fazli impregnated with emulsions of oleoresin from ginger (Zingiber officinale) to provide spicy flavor and antioxidant and anti-inflammatory properties; when comparing the product with a commercial one of similar characteristics, they concluded that osmotic pretreatment improved the retention of vitamin C, total phenols and β-carotene by 60.2, 76.8 and 85.6%, respectively; in addition, they reduced the total processing time by up to 376.7%.
Authors such as Aguirre-García et al. (2020) impregnated antioxidant compounds of beet juice in slices of apple cv. Granny Smith, achieving an increase of up to 46 and 115% in total phenol content and antioxidant capacity, respectively, compared to the fresh product. Meanwhile, Barragán-Iglesias et al. (2018) dehydrated cubes of papaya (Carica papaya L. cv. Maradol) impregnated with calcium hydroxide (Ca(OH)2), resulting in a product with a firm exterior and soft interior and reduced shrinkage and deformation, they also decreased drying time by up to 37%.
Lovera et al. (2018) studied the effect of impregnation of calcium lactate (C6H10CaO6) and calcium gluconate (C12H22CaO14) in papaya cylinders on the freezing time of the product, their results show that the OD allowed reducing the time and costs of production since the freezing time of the fruit in fresh, impregnated with calcium and osmo-dehydrated was 23, 17 and 5 min in tunnel, and 118, 83 and 60 min in a domestic freezer, respectively.
Since OD is very versatile, it has often been combined with other processing techniques in order to increase the mass transfer rate. In this sense, the application of high hydrostatic pressure (HP) generates compression and decompression of the product and leads to cell disintegration, which increases the cell permeabilization index (Zp) and mass flow during the osmotic process.
Research carried out by George et al. (2016) osmo-dehydrated slices of apple cv. Fuji with sucrose solutions and extract of kokum (Garcinia indica), their results showed a significant increase in the impregnation of kokum antioxidants in apples treated in a combination of HP and OD, compared to the product that was only processed by OD.
The OD has also been coupled with ultrasound (US), a process characterized by a vibration of air in a frequency range (20-100 MHz), and they have two alternating mechanisms: cavitation-compression and expansion, which cause erosion and decomposition of surface particles of the product, and lead to the formation of microscopic channels.
The use of US prior to OD has been studied by Maleki et al. (2020), who dehydrated carrot slices impregnated with phenolic compounds from the flower of roselle (Hibiscus sabdariffa), their results show that the maximum impregnation of phenolic compounds in the product was without US, this was attributed to prolonged US times damaging the microchannels of plant tissue and decreasing mass flow during OD. Subatmospheric pressures for a short time (5 to 20 min) have also been used, a technique known as vacuum impregnation (VI) and which, in combination with OD, is called pulsed vacuum osmotic dehydration (PVOD).
In Figure 4, it was observed how in the PVOD, the gas occluded in the pores of the plant tissue was eliminated until mechanical equilibrium was achieved, where once the atmospheric pressure is restored, the pores are filled with the external solution, which generates an increase in the product-hypertonic solution contact surface within the intercellular spaces of the plant tissue, and it causes structural modifications of the OD (Figure 4B), to a greater number of internal cells (Figure 4C), mainly in the outer cells of the product.
Through VI and PVOD, various fruit and vegetable products impregnated with bioactive compounds have been formulated (Table 1). The PVOD has been widely used to enrich plant matrices with water-soluble bioactive compounds (antioxidants, water-soluble vitamins, minerals, etc).
| Functional ingredient | Plant matrix | Reference |
|---|---|---|
| Minerals | Pineapple triangles enriched by VI with up to 24 487.3 μg g-1 (d.b.) of calcium chloride dihydrate. | Mateus de Lima et al. (2016) |
| Vitamins | Diacol Capiro potato slices enriched by VI with up to 72 and 53% vitamin E and C, respectively. | Duarte-Correa et al. (2020) |
| Antioxidants | Tommy Atkins mango slices impregnated with up to 3.34 mg GAE g-1 dm of grape extract polyphenols by PVOD. Alba strawberry halves enriched by VI with up to 22% cranberry juice polyphenols. | Batista de Medeiros et al. (2019) Tylewicz et al. (2019) |
| Probiotics | Royal Gala apple cubes impregnated by OD with up to 7 to 8 log10 (CFU g-1 db.) of Lactobacillus plantarum. Tabasco banana slices impregnated by OD and PVOD with up to 6 to 7 log10 (CFU g-1 db.) with Lactobacillus rhamnosus. ‘Granny Smith’ apple rings impregnated by VI with up to 7 to 8 log10 (CFU g-1 db.) with Lactobacillus salivarius spp. salivarius. | Emser et al. (2017) Huerta-Vera et al. (2017) Burca-Busaga et al. (2020) |
| Enzymes | ‘Kent’ mango cubes impregnated by PVOD with pectin methylesterase (PME), which improved the firmness of the product. | Sulistyawati et al. (2018) |
On the other hand, it has been possible to impregnate with viable probiotic bacteria such as Lactobacillus (L. plantarum, L. rhamnosus, L. salivarius, L. casei, among others) with and without barrier technologies against the effects of gastric juice and bile acid from the stomach. Emulsions (micro and nanoemulsions) have even been used as a strategy that allows the incorporation of bioactive compounds of lipophilic nature (fat-soluble vitamins, essential oils, oleoresins) with application limited to products rich in fats and oils in water-rich foods such as fruits and vegetables.
The application of PVOD has been studied in various plant matrices and it has been observed that an important factor in the impregnation of solutes in plant tissue is its ɛe.
Some studies have evaluated the physicochemical stability during the storage of osmo-dehydrated fruit and vegetable products impregnated with functional ingredients. The enrichment of plant matrices with compounds with antioxidant capacity prevents oxidative stress and damage to cellular structures (DNA, membrane proteins and lipids) that are related to neurodegenerative diseases.
Osmo-dehydrated cylinders of apple cv. Braeburn in solutions of sucrose and concentrated juice from aronia berries (Aronia melanocarpa) (120 min at 40 and 60 °C) and then dried them by lyophilization or convective air, and finally, vacuum packed in polyethylene bags to be stored (25, 35, and 45 °C) for 7 and 12 months (Cichowska and Kowalska, 2018). The results indicated that the product showed microbiological stability during and after storage regardless of the drying method, in addition, osmotic pretreatment induced a thermoprotective effect on the color of the product during storage.
Osmo-dehydrated slices of cucumber (Cucumis sativus) with hypertonic solutions of glycerol in combination with 20 different herbal infusions [chamomile (Matricaria chamomilla L.), oregano (Origanum vulgare), mint (Mentha pulegium L.), jasmine flowers (Jasminum officinale), turmeric (Curcuma longa)] rich in phenolic compounds and evaluated their quality during storage (37 °C), resulting in the osmo-treated product having a color, texture and microbiological evaluation two to almost four times higher than the untreated product (Giannakourou et al., 2019).
On the other hand, the enrichment of plant matrices with probiotics has been evaluated, which are live microorganisms that, when consumed in adequate quantities, inhibit the proliferation of pathogenic bacteria, improve the digestive process and the acquired immune response (Rascón et al., 2018).
Osmo-dehydrated cubes of apple var. Royal Gala with sucrose or sorbitol solutions (40 and 60 °Brix) containing L. plantarum, the apple cubes were stored (4 °C for 6 days) and evaluated by digestive simulation, their results exhibit a probiotic viability of 7 to 8 log10 (CFU g-1) during storage with a survival of 7 log10 (CFU g-1) during digestive simulation (Emser et al., 2017).
lyophilized slices of banana var. Tabasco enriched with L. rhamnosus using a sucrose solution (50 °Brix) and evaluated the effect of aw (0.115 to 0.846) on the probiotic stability of the product during storage (25 °C for 42 days), their results show a bacterial viability of 6 to 7 log10 (CFU g-1 db) at low water activities (0.115 to 0.329) for a maximum period of 28 days (Rascón et al., 2018).
In addition to bioactive compounds, OD has also been useful in incorporating antimicrobial agents and flavor additives into plant matrices. Akharume et al. (2018) dried by convective air slices of apple var. Golden Delicious previously osmo-dehydrated with a solution of sucrose (42 °Brix) and refined liquid smoke and evaluated their color, texture, and microbial load during storage (150 days) in polyethylene bags with and without vacuum, their results show that apples with osmotic pretreatment with refined liquid smoke presented a characteristic brown coloration, better texture properties and a significant microbial reduction during storage compared to slices of apples dried by convection without osmotic pretreatment.
Studies show that through OD, with or without the implementation of other preservation techniques, it is possible to obtain fruit and vegetable products minimally processed and enriched with bioactive compounds with acceptable and sufficient physicochemical stability to exert a beneficial effect on the consumer.
However, fruit and vegetable products enriched with bioactive components by OD still face technological challenges and research opportunities that include: demonstrating their physicochemical stability during storage and the mechanisms of absorption and metabolism of the active ingredient in biological models. In addition, it is necessary to develop global policies that regulate and supervise the production and sale of functional foods in order to protect consumers from buying products with alleged attributions or properties beneficial to their health.
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